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TWEAK/TNFSF12 Active Protein | TNFSF12 active protein
TWEAK/TNFSF12 Protein, Rat, Recombinant (hFc Tag)
Gene Names
Tnfsf12; TWEAK; Tnlg4a
Purity
>85% as determined by SDS-PAGE
Synonyms
TWEAK/TNFSF12; TWEAK/TNFSF12 Protein; Rat; Recombinant (hFc Tag); Rat TNFSF12 Protein (Fc Tag); tumor necrosis factor (ligand) superfamily; member 12; TNFSF12 active protein
Host
HEK293 Cells
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Arg105-His249
Species
Rat
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu
Tag
N-human IgG1-Fc
Protein Construction
A DNA sequence encoding the rat TNFSF12 (NP_001001513.2)(Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
Immobilized Fc-rat TNFSF12 at 10ug/ml (100uL/well) can bind biotinylated human Fc-TNFRSF12A. The EC50 of biotinylated human Fc-TNFRSF12A is 0.12-0.28ug/ml.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for TNFSF12 active protein
Background: TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.
Product Categories/Family for TNFSF12 active protein
References
Wiley SR, et al. (2004) TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cytokine Growth Factor Rev. 14(3-4):241-9.
Campbell S, et al. (2006) The role of TWEAK/Fn14 in the pathogenesis of inflammation and systemic autoimmunity. Front Biosci. 9:2273-84.
Lynch CN, et al. (1999) TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 274(13):8455-9.
Campbell S, et al. (2006) The role of TWEAK/Fn14 in the pathogenesis of inflammation and systemic autoimmunity. Front Biosci. 9:2273-84.
Lynch CN, et al. (1999) TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 274(13):8455-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,381 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor superfamily member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
TWEAK; Tnlg4a
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12
UniProt Protein Name
Protein Tnfsf12
Protein Family
UniProt Gene Name
Prmt2
UniProt Entry Name
Q6AYC1_RAT
Research Articles on TNFSF12
1. Endurance training prevents TWEAK but not myostatin-mediated cardiac remodelling in cancer cachexia
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Product Notes
The TNFSF12 prmt2 (Catalog #AAA8123363) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Arg105-His 249. It is sometimes possible for the material contained within the vial of "TWEAK/TNFSF12, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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