CRABP2 recombinant protein

CRABP2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122804

• Gene Names: Crabp2; Crabp-2; CrabpII; AI893628
• Purity: >96% as determined by SDS-PAGE
• Synonyms: CRABP2; CRABP2 Protein; Mouse; Recombinant (His Tag); Mouse CRABP2/CRABPII Protein (His Tag); AI893628 Protein; Crabp-2 Protein; CrabpII Protein; cellular retinoic acid binding protein 2; CRABP2 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro2-Glu138

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse CRABP2 (P22935)(Pro 2-Glu 138) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CRABP2 recombinant protein

Background: Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to thecalycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.

References

Deak,KL.et al., 2005,Birth Defects Res A Clin Mol Teratol.73 (11): 868-75. Plantier, L. et al., 2008, Thorax 63 (11): 1012-7. Calmon, M.F. et al., 2009, Neoplasia 11 (12): 1329-39. Welch, I.D. et al., 2009, Arthritis Res Ther 11 (1): R14.

NCBI and Uniprot Product Information

• NCBI GI #: 33469075
• NCBI GeneID: 12904
• NCBI Accession #: NP_031785.1
• NCBI GenBank Nucleotide #: NM_007759.2
• UniProt Accession #: P22935
• Molecular Weight: 15,746 Da
• NCBI Official Full Name: cellular retinoic acid-binding protein 2
• NCBI Official Synonym Full Names: cellular retinoic acid binding protein II
• NCBI Official Symbol: Crabp2
• NCBI Official Synonym Symbols: Crabp-2; CrabpII; AI893628
• NCBI Protein Information: cellular retinoic acid-binding protein 2; CRABP-II; cellular retinoic acid-binding protein II
• UniProt Protein Name: Cellular retinoic acid-binding protein 2
• Protein Family: Cellular retinoic acid-binding protein
• UniProt Gene Name: Crabp2
• UniProt Synonym Gene Names: CRABP-II
• UniProt Entry Name: RABP2_MOUSE