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SDS-PAGE

CRABP2 recombinant protein

Recombinant Human CRABP2 Protein (His tag)

Gene Names
CRABP2; RBP6; CRABP-II
Purity
> 96 % as determined by SDS-PAGE
Synonyms
CRABP2; Recombinant Human CRABP2 Protein (His tag); RP11-66D17.4; CRABP-II; RBP6; CRABP2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 96 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Sequence Length
138
Application Notes
The recombinant human CRABP2 consisting of 144 amino acids and migrates as an approximately 16.5 kDa band in SDS-PAGE under reducing conditions as predicted.
Predicted N Terminal
Met
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for CRABP2 recombinant protein
Background: Mouse cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.

Description: A DNA sequence encoding the human CRABP2 (NP_001869.1) (Pro2-Glu 138) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,693 Da
NCBI Official Full Name
cellular retinoic acid-binding protein 2
NCBI Official Synonym Full Names
cellular retinoic acid binding protein 2
NCBI Official Symbol
CRABP2
NCBI Official Synonym Symbols
RBP6; CRABP-II
NCBI Protein Information
cellular retinoic acid-binding protein 2
UniProt Protein Name
Cellular retinoic acid-binding protein 2
UniProt Gene Name
CRABP2
UniProt Synonym Gene Names
CRABP-II

NCBI Description

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

CRABP2: Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: protein binding; retinoid binding

Biological Process: epidermis development; regulation of transcription, DNA-dependent; signal transduction

Research Articles on CRABP2

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Product Notes

The CRABP2 crabp2 (Catalog #AAA2545869) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CRABP2 consisting of 144 amino acids and migrates as an approximately 16.5 kDa band in SDS-PAGE under reducing conditions as predicted. Researchers should empirically determine the suitability of the CRABP2 crabp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRABP2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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