Immunohistochemistry (IHC)
(Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Mouse CRABP2 Polyclonal Antibody | anti-CRABP2 antibody
Anti-CRABP2 Antibody, Rabbit Polyclonal
Gene Names
Crabp2; Crabp-2; CrabpII; AI893628
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
CRABP2; Polyclonal Antibody; Anti-CRABP2 Antibody; Rabbit Polyclonal; CRABP2 Antibody; Rabbit PAb; Antigen Affinity Purified; cellular retinoic acid binding protein 2; Anti-AI893628 Antibody; Anti-Crabp-2 Antibody; Anti-CrabpII Antibody; anti-CRABP2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse CRABP2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CRABP2 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
Immunogen
Recombinant Mouse CRABP2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CRABP2 (rM CRABP2). CRABP2 specific IgG was purified by Mouse CRABP2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CRABP2 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CRABP2 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CRABP2 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of mCRABP2 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse mCRABP2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of mCRABP2 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse mCRABP2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Related Product Information for anti-CRABP2 antibody
Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to thecalycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.
References
Deak, KL.et al., 2005, Birth Defects Res A Clin Mol Teratol.73 (11): 868-75. Plantier, L. et al., 2008, Thorax 63 (11): 1012-7. Calmon, M.F. et al., 2009, Neoplasia 11 (12): 1329-39. Welch, I.D. et al., 2009, Arthritis Res Ther 11 (1): R14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,746 Da
NCBI Official Full Name
cellular retinoic acid-binding protein 2
NCBI Official Synonym Full Names
cellular retinoic acid binding protein II
NCBI Official Symbol
Crabp2
NCBI Official Synonym Symbols
Crabp-2; CrabpII; AI893628
NCBI Protein Information
cellular retinoic acid-binding protein 2; CRABP-II; cellular retinoic acid-binding protein II
UniProt Protein Name
Cellular retinoic acid-binding protein 2
Protein Family
UniProt Gene Name
Crabp2
UniProt Synonym Gene Names
CRABP-II
UniProt Entry Name
RABP2_MOUSE
Similar Products
Product Notes
The CRABP2 crabp2 (Catalog #AAA8111711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CRABP2 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CRABP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:5000-1:10,000 IHC-P: 1:200-1:1000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the CRABP2 crabp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRABP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
