CENPB recombinant protein

CENPB Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8122106

• Purity: >95% as determined by SDS-PAGE
• Synonyms: CENPB; CENPB Protein; Human; Recombinant (His Tag); Human CENPB Protein (His Tag); CENPB recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM PB, 15mM NaCl, 10% glycerol, pH7.0, 0.5mM TCEP. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser599

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CENPB (NP_001801.1)(Met1-Ser599) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21735415
• NCBI GeneID: 1059
• NCBI Accession #: NP_001801.1
• NCBI GenBank Nucleotide #: NM_001810.5
• UniProt Accession #: P07199; Q96EI4
• NCBI Official Full Name: major centromere autoantigen B
• NCBI Official Synonym Full Names: centromere protein B, 80kDa
• NCBI Official Symbol: CENPB
• NCBI Protein Information: major centromere autoantigen B; CENP-B
• UniProt Protein Name: Major centromere autoantigen B
• Protein Family: CENPB DNA-binding domain-containing protein
• UniProt Gene Name: CENPB
• UniProt Synonym Gene Names: CENP-B
• UniProt Entry Name: CENPB_HUMAN

NCBI Description

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: chromosome; nucleus; chromosome, pericentric region

Molecular Function: centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding

Biological Process: regulation of transcription, DNA-dependent