CLEC10A recombinant protein

CLEC10A Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8120502

• Gene Names: CLEC10A; HML; MGL; HML2; CD301; CLECSF13; CLECSF14
• Purity: >85% as determined by SDS-PAGE
• Synonyms: CLEC10A; CLEC10A Protein; Human; Recombinant (ECD; hFc Tag); Human CLEC10A/MGL1/CD301 Protein (ECD; Fc Tag); CD301 Protein; CLECSF13 Protein; CLECSF14 Protein; HML Protein; HML2 Protein; MGL Protein; C-type lectin domain family 10; member A; CLEC10A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln61-His292

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CLEC10A (Q8IUN9-2)(Gln61-His292) was expressed, with the fused Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLEC10A recombinant protein

Background: CLEC10A, also known as the macrophage galactose-type calcium-type lectins (MGLs; CD301) constitute a unique class of C-type lectins because of their specificity for galactose and its structural homologues. MGLs/CD301 is a type II transmembrane glycoproteins and is expressed on macrophages and related cells of myeloid origins, particularly immature dendritic cells (DCs). There are 2 homologues: MGL1 and MGL2 (CD301a and CD301b) in mice. MGL1/CD301a induces both the production and secretion of interleukin (IL)-10. MGL1/CD301a plays a protective role against colitis by effectively inducing IL-10 production by colonic lamina propria macrophages in response to invading commensal bacteria.

References

Saba K, et al. (2009) A C-type lectin MGL1/CD301a plays an anti-inflammatory role in murine experimental colitis. Am J Pathol. 174(1): 144-52.Suzuki N, et al. (2002) Molecular cloning and expression of cDNA encoding human macrophage C-type lectin: its unique carbohydrate binding specificity for Tn antigen. J Immunol. 1996 ;156: 128-135.Tsuiji M, et al. (2002) Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1. J Biol Chem. 277: 28892-28901.Denda-Nagai K, et al. (2002) Macrophage C-type lectin on bone marrow-derived immature dendritic cells is involved in the internalization of glycosylated antigens. Glycobiology. 12: 443-450.

NCBI and Uniprot Product Information

• NCBI GI #: 59797948
• NCBI GeneID: 10462
• UniProt Accession #: Q8IUN9; Q14538; Q6PIW3; A8K8J8
• Molecular Weight: 316
• NCBI Official Full Name: C-type lectin domain family 10 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 10, member A
• NCBI Official Symbol: CLEC10A
• NCBI Official Synonym Symbols: HML; MGL; HML2; CD301; CLECSF13; CLECSF14
• NCBI Protein Information: C-type lectin domain family 10 member A; macrophage lectin 2 (calcium dependent); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13 (macrophage-derived); C-type (calcium dependent, carbohydrate-recognition domain) l
• UniProt Protein Name: C-type lectin domain family 10 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC10A
• UniProt Synonym Gene Names: CLECSF13; CLECSF14; HML
• UniProt Entry Name: CLC10_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CLEC10A: Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbohydrate binding

Biological Process: innate immune response; endocytosis