Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-3 adrenergic receptor (ADRB3) Standard Curve.)
Human Beta-3 Adrenergic Receptor (ADRB3) ELISA Kit | ADRB3 elisa kit
Human Beta-3 Adrenergic Receptor (ADRB3) ELISA Kit
Gene Names
ADRB3; BETA3AR
Reactivity
Human
Synonyms
Beta-3 Adrenergic Receptor (ADRB3); Human Beta-3 Adrenergic Receptor (ADRB3) ELISA Kit; ADRB3; BETA3AR; FLJ99960; ADRB3 elisa kit
Reactivity
Human
Specificity
Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADRB3. No significant cross-reactivity or interference between Human ADRB3 and analogues was observed.
Sequence Length
408
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-3 adrenergic receptor (ADRB3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-3 adrenergic receptor (ADRB3) Standard Curve.)
Related Product Information for ADRB3 elisa kit
Description: This Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRB3 in samples. An antibody specific for ADRB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADRB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ADRb3 is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Some beta3 agonists have demonstrated antistress effects in animal studies, suggesting it also has a role in the CNS. Beta3-Receptors are found in the gallbladder and in brain adipose tissue. Their role in gallbladder physiology is unknown, but they are thought to play a role in lipolysis and thermogenesis in brown fat. Beta adrenergic receptors are involved in the epinephrine- and norepinephrine-induced activation of adenylate cyclase through ngono the action of the G proteins of the type Gs.SR 59230A was thought to be a selective beta3 antagonist but later found to also be an antagonist of the alpha1 receptor.
Background: ADRb3 is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Some beta3 agonists have demonstrated antistress effects in animal studies, suggesting it also has a role in the CNS. Beta3-Receptors are found in the gallbladder and in brain adipose tissue. Their role in gallbladder physiology is unknown, but they are thought to play a role in lipolysis and thermogenesis in brown fat. Beta adrenergic receptors are involved in the epinephrine- and norepinephrine-induced activation of adenylate cyclase through ngono the action of the G proteins of the type Gs.SR 59230A was thought to be a selective beta3 antagonist but later found to also be an antagonist of the alpha1 receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
beta-3 adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor beta 3
NCBI Official Symbol
ADRB3
NCBI Official Synonym Symbols
BETA3AR
NCBI Protein Information
beta-3 adrenergic receptor
UniProt Protein Name
Beta-3 adrenergic receptor
Protein Family
UniProt Gene Name
ADRB3
UniProt Synonym Gene Names
ADRB3R; B3AR; Beta-3 adrenoceptor
UniProt Entry Name
ADRB3_HUMAN
NCBI Description
The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq, Feb 2009]
Research Articles on ADRB3
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Product Notes
The Human ADRB3 adrb3 (Catalog #AAA9714616) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714616 ELISA Kit recognizes Human ADRB3. It is sometimes possible for the material contained within the vial of "Beta-3 Adrenergic Receptor (ADRB3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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