CLEC10A / MGL1 / CD301 recombinant protein

Recombinant Human CLEC10A / MGL1 / CD301 Protein (Fc tag)

Cat. Num. AAA2546066

• Gene Names: CLEC10A; HML; MGL; HML2; CD301; CLECSF13; CLECSF14
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CLEC10A / MGL1 / CD301; Recombinant Human CLEC10A / MGL1 / CD301 Protein (Fc tag); CLEC10A; CLECSF13; CLECSF14; HML; CLEC10A / MGL1 / CD301 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 292

• Application Notes: The recombinant human CLEC10A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 492 amino acids and has a predicted molecular mass of 54.6 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Glu
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CLEC10A / MGL1 / CD301 recombinant protein

Background: CLEC10A, also known as the macrophage galactose-type calcium-type lectins (MGLs; CD301) constitute a unique class of C-type lectins because of their specificity for galactose and its structural homologues. MGLs/CD301 is a type II transmembrane glycoproteins and is expressed on macrophages and related cells of myeloid origins, particularly immature dendritic cells (DCs). There are 2 homologues: MGL1 and MGL2 (CD301a and CD301b) in mice. MGL1/CD301a induces both the production and secretion of interleukin (IL)-10. MGL1/CD301a plays a protective role against colitis by effectively inducing IL-10 production by colonic lamina propria macrophages in response to invading commensal bacteria.

Description: A DNA sequence encoding the human CLEC10A (Q8IUN9-2) (Gln61-His292) was expressed, with the fused Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 53832016
• NCBI GeneID: 10462
• NCBI Accession #: NP_006335.2
• NCBI GenBank Nucleotide #: NM_006344.3
• UniProt Accession #: Q8IUN9; Q14538; Q6PIW3; A8K8J8
• Molecular Weight: 28,877 Da
• NCBI Official Full Name: C-type lectin domain family 10 member A isoform 2
• NCBI Official Synonym Full Names: C-type lectin domain family 10 member A
• NCBI Official Symbol: CLEC10A
• NCBI Official Synonym Symbols: HML; MGL; HML2; CD301; CLECSF13; CLECSF14
• NCBI Protein Information: C-type lectin domain family 10 member A
• UniProt Protein Name: C-type lectin domain family 10 member A
• UniProt Gene Name: CLEC10A
• UniProt Synonym Gene Names: CLECSF13; CLECSF14; HML

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CLEC10A: Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: plasma membrane