ICAM-2 Recombinant Protein | ICAM2 recombinant protein

ICAM-2 Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA8118604

• Gene Names: ICAM2; CD102
• Purity: >97 % as determined by SDS-PAGE
• Synonyms: ICAM-2; ICAM-2 Protein; Human; Recombinant (His & hFc Tag); Human ICAM-2/CD102 Protein (His & Fc Tag); intercellular adhesion molecule 2; CD102 Protein; ICAM2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 449aa
• Sequence: Met1-Gln223

• Species: Human
• Tag: His & hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser22
• Protein Construction: |A DNA sequence encoding the extracellular domain (Met 1-Gln 223) of human ICAM2 (NP_000864.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus
• Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to ICAM2-coated plates (12.5ug/mL, 100uL/well), approximately 50 %-60 % will adhere specifically.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for ICAM2 recombinant protein

Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As the second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte with other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity, and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relationship with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in the identification of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

References

Weber KS, et al. (2004) Sialylation of ICAM-2 on platelets impairs adhesion of leukocytes via LFA-1 and DC-SIGN. Inflammation. 28(4): 177-88.Tanaka H, et al. (2004) ICAM-2 gene therapy for peritoneal dissemination of scirrhous gastric carcinoma. Clin Cancer Res. 10(14): 4885-92.Younis AI, et al. (2005) Intercellular adhesion molecule-2 (ICAM-2) in experimental trichinosis. J Egypt Soc Parasitol. 35(3): 1019-26.

NCBI and Uniprot Product Information

• NCBI GI #: 153082696
• NCBI GeneID: 3384
• NCBI Accession #: NP_000864.2
• NCBI GenBank Nucleotide #: NM_000873.3
• UniProt Accession #: P13598; Q14600
• Molecular Weight: 30,654 Da
• NCBI Official Full Name: intercellular adhesion molecule 2
• NCBI Official Synonym Full Names: intercellular adhesion molecule 2
• NCBI Official Symbol: ICAM2
• NCBI Official Synonym Symbols: CD102
• NCBI Protein Information: intercellular adhesion molecule 2; ICAM-2
• UniProt Protein Name: Intercellular adhesion molecule 2
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM2
• UniProt Synonym Gene Names: ICAM-2
• UniProt Entry Name: ICAM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM2: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: membrane; integral to plasma membrane; plasma membrane; uropod

Molecular Function: integrin binding

Biological Process: cell-cell adhesion; regulation of immune response; extracellular matrix organization and biogenesis; cell adhesion