ICAM-2 / CD102 recombinant protein

Recombinant Human ICAM-2 / CD102 Protein (His & Fc tag)

Cat. Num. AAA2546330

• Gene Names: ICAM2; CD102
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ICAM-2 / CD102; Recombinant Human ICAM-2 / CD102 Protein (His & Fc tag); CD102; ICAM2; ICAM-2 / CD102 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 275

• Application Notes: The recombinant human ICAM2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 449 amino acids and has a predicted molecular mass of 50.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhICAM2/Fc monomer is approximately 85-95 kDa due to glycosylation.
• Predicted N Terminal: Ser 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ICAM-2 / CD102 recombinant protein

Background: Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in identification of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

Description: A DNA sequence encoding the extracellular domain (Met 1-Gln 223) of human ICAM2 (NP_000864.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 153082696
• NCBI GeneID: 3384
• NCBI Accession #: NP_000864.2
• NCBI GenBank Nucleotide #: NM_000873.3
• UniProt Accession #: P13598; Q14600
• Molecular Weight: 30,654 Da
• NCBI Official Full Name: intercellular adhesion molecule 2
• NCBI Official Synonym Full Names: intercellular adhesion molecule 2
• NCBI Official Symbol: ICAM2
• NCBI Official Synonym Symbols: CD102
• NCBI Protein Information: intercellular adhesion molecule 2
• UniProt Protein Name: Intercellular adhesion molecule 2
• UniProt Gene Name: ICAM2
• UniProt Synonym Gene Names: ICAM-2

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM2: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: membrane; plasma membrane

Molecular Function: integrin binding

Biological Process: cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; stimulatory C-type lectin receptor signaling pathway