MIG/CXCL9 ELISA Pair Set | CXCL9 elisa pair set

MIG/CXCL9 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118318

• Synonyms: MIG/CXCL9; MIG/CXCL9 Matched ELISA Antibody Pair Set; Human; Human CXCL9 ELISA Pair Set; chemokine (C-X-C motif) ligand 9; CMK Matched ELISA Antibody Pair Set; crg-10 Matched ELISA Antibody Pair Set; Humig Matched ELISA Antibody Pair Set; MIG Matched ELISA Antibody Pair Set; SCYB9 Matched ELISA Antibody Pair Set; CXCL9 elisa pair set

• Specificity: Quantitative determination of Human MIG/CXCL9

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 9.38-600pg/mL
• Sensitivity: 9.38pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Human CXCL9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 0.5ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human CXCL9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.15ug/mL in Dilution Buffer before use. ; 3. Standard Each vial contains 13 ng of recombinant Human CXCL9. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 600 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CXCL9 elisa pair set

Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as Monokine induced by gamma interferon (MIG), is a small cytokine belonging to the CXC chemokine family. The function of this chemokine has not been specifically defined; however, it is thought to be involved in T cell trafficking. CXCL9/MIG functions as one of the three ligands of chemokine receptor CXCR3 which is a G protein-coupled receptor found predominantly on T cells. CXCL9/MIG, together with CXCL10 and CXCL11, may activate CXCR3 by binding to it. CXCL9 serves as a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. It has been observed that tumour endothelial cells secrete high levels of CXCL9 in all, and CXCL10 in most melanoma metastases. Experiment data represent novel mechanisms by which tumour cells in melanoma metastases might use the chemokine-expressing endothelium to leave the tumour and eventually to form additional metastases at distinct sites. Experiment results also improved that CXCL9/MIG plays an important role in CD4+ T lymphocyte recruitment and development of CAV, MOMA-2+ macrophages are the predominant recipient-derived source of CXCL9/MIG, and recipient CD4 lymphocytes are necessary for sustained CXCL9/MIG production and CAV development in this model. Neutralization of the chemokine CXCL9/MIG may have therapeutic potential for the treatment of chronic rejection after heart transplantation.

This MIG/CXCL9 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human MIG/CXCL9. It contains Human MIG/CXCL9 capture antibody, Human MIG/CXCL9 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human MIG/CXCL9 protein.

References

Ruehlmann JM, et al. (2001) MIG (CXCL9) chemokine gene therapy combines with antibody-cytokine fusion protein to suppress growth and dissemination of murine colon carcinoma. Cancer Res. 61(23): 8498-503.Belperio JA, et al. (2003) Role of CXCL9/CXCR3 chemokine biology during pathogenesis of acute lung allograft rejection. J Immunol. 171(9): 4844-52.Colvin RA, et al. (2004) Intracellular domains of CXCR3 that mediate CXCL9, CXCL10, and CXCL11 function. J Biol Chem. 279(29): 30219-27.Valbuena G, et al. (2003) Expression analysis of the T-cell-targeting chemokines CXCL9 and CXCL10 in mice and humans with endothelial infections caused by rickettsiae of the spotted fever group. Am J Pathol. 163(4): 1357-69.