MIG/CXCL9 Recombinant Protein | CXCL9 recombinant protein

MIG/CXCL9 Protein, Mouse, Recombinant

Cat. Num. AAA8122568

• Gene Names: Cxcl9; CMK; Mig; MuMIG; Scyb9; crg-10; BB139920
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MIG/CXCL9; MIG/CXCL9 Protein; Mouse; Recombinant; Mouse CXCL9/MIG/C-X-C motif chemokine 9 Protein; BB139920 Protein; CMK Protein; crg-10 Protein; Mig Protein; MuMIG Protein; Scyb9 Protein; chemokine (C-X-C motif) ligand 9; CXCL9 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr22-Thr126

• Species: Mouse
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of mouse CXCL9 (P18340)(Thr 22-Thr 126) was expressed, with an initial Met at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CXCL9 recombinant protein

Background: Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as Monokine induced by gamma interferon (MIG), is a small cytokine belonging to the CXC chemokine family. The function of this chemokine has not been specifically defined; however, it is thought to be involved in T cell trafficking. CXCL9/MIG functions as one of the three ligands of chemokine receptor CXCR3 which is a G protein-coupled receptor found predominantly on T cells. CXCL9/MIG, together with CXCL10 and CXCL11, may activate CXCR3 by binding to it. CXCL9 serves as a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. It has been observed that tumour endothelial cells secrete high levels of CXCL9 in all, and CXCL10 in most melanoma metastases. Experiment data represent novel mechanisms by which tumour cells in melanoma metastases might use the chemokine-expressing endothelium to leave the tumour and eventually to form additional metastases at distinct sites. Experiment results also improved that CXCL9/MIG plays an important role in CD4+ T lymphocyte recruitment and development of CAV, MOMA-2+ macrophages are the predominant recipient-derived source of CXCL9/MIG, and recipient CD4 lymphocytes are necessary for sustained CXCL9/MIG production and CAV development in this model. Neutralization of the chemokine CXCL9/MIG may have therapeutic potential for the treatment of chronic rejection after heart transplantation.

References

Ruehlmann JM, et al. (2001) MIG (CXCL9) chemokine gene therapy combines with antibody-cytokine fusion protein to suppress growth and dissemination of murine colon carcinoma. Cancer Res. 61(23): 8498-503.Belperio JA, et al. (2003) Role of CXCL9/CXCR3 chemokine biology during pathogenesis of acute lung allograft rejection. J Immunol. 171(9): 4844-52.Colvin RA, et al. (2004) Intracellular domains of CXCR3 that mediate CXCL9, CXCL10, and CXCL11 function. J Biol Chem. 279(29): 30219-27.Valbuena G, et al. (2003) Expression analysis of the T-cell-targeting chemokines CXCL9 and CXCL10 in mice and humans with endothelial infections caused by rickettsiae of the spotted fever group. Am J Pathol. 163(4): 1357-69.

NCBI and Uniprot Product Information

• NCBI GI #: 110626107
• NCBI GeneID: 17329
• NCBI Accession #: NP_032625.2
• NCBI GenBank Nucleotide #: NM_008599.4
• UniProt Accession #: P18340; Q8C9J0; Q99J60
• Molecular Weight: 14,444 Da
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 9
• NCBI Official Symbol: Cxcl9
• NCBI Official Synonym Symbols: CMK; Mig; MuMIG; Scyb9; crg-10; BB139920
• NCBI Protein Information: C-X-C motif chemokine 9; M119; gamma interferon-induced monokine; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma; protein m119; small inducible cytokine B subfamily (Cys-X-Cys), member 9; smal
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl9
• UniProt Synonym Gene Names: Mig; Scyb9; MIG; MuMIG
• UniProt Entry Name: CXCL9_MOUSE

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; immune response; chemotaxis; inflammatory response; defense response to virus; positive regulation of myoblast differentiation; regulation of cell proliferation