Syndecan-1/CD138 Cell Lysate | SDC1 cell lysate
Rat Syndecan-1/CD138 HEK293 Overexpression Lysate
Gene Names
Sdc1; HSPG; Synd1; SYNDECA; Syndecan
Applications
Western Blot
Synonyms
Syndecan-1/CD138; Rat Syndecan-1/CD138 HEK293 Overexpression Lysate; Rat Syndecan-1/SDC1/CD138 HEK293 Cell Lysate (WB positive control); syndecan 1; SDC1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
472aa
Sequence
Met1-Lys253
Applicable Applications for SDC1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Rat
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the rat SDC1 (P26260) (Met1-Lys253) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SDC1 cell lysate
Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.
This Rat Syndecan-1/CD138 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Syndecan-1/CD138 protein from the overexpression lysate was verified.
This Rat Syndecan-1/CD138 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Syndecan-1/CD138 protein from the overexpression lysate was verified.
References
Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,214 Da
NCBI Official Full Name
syndecan-1
NCBI Official Synonym Full Names
syndecan 1
NCBI Official Symbol
Sdc1
NCBI Official Synonym Symbols
HSPG; Synd1; SYNDECA; Syndecan
NCBI Protein Information
syndecan-1
UniProt Protein Name
Syndecan-1
Protein Family
UniProt Gene Name
Sdc1
UniProt Synonym Gene Names
Synd1; SYND1
UniProt Entry Name
SDC1_RAT
NCBI Description
mouse homolog promotes cell-cell adhesion [RGD, Feb 2006]
Uniprot Description
syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.
Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Membrane protein, integral
Cellular Component: cell surface; cytoplasm; external side of plasma membrane; focal adhesion; integral to membrane; protein complex
Molecular Function: extracellular matrix binding; glycoprotein binding; protein binding; protein C-terminus binding
Biological Process: cell adhesion; cell migration; cell-cell signaling; inflammatory response; myoblast development; odontogenesis; response to calcium ion; response to cAMP; response to glucocorticoid stimulus; response to hydrogen peroxide; response to organic substance; response to toxin; Sertoli cell development; striated muscle cell development; ureteric bud development; Wnt receptor signaling pathway through beta-catenin; wound healing
Research Articles on SDC1
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Product Notes
The SDC1 sdc1 (Catalog #AAA8117577) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 472aa. AAA Biotech's Syndecan-1/CD138 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SDC1 sdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys25 3. It is sometimes possible for the material contained within the vial of "Syndecan-1/CD138, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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