CD59 cell lysate
Rat CD59 HEK293 Overexpression Lysate
Gene Names
Cd59; Cd59a; Cd59b; MACIF; MACIP; MAC-IP
Applications
Western Blot
Synonyms
CD59; Rat CD59 HEK293 Overexpression Lysate; Rat CD59/CD59A/MAC/IP HEK293 Cell Lysate (WB positive control); CD59 molecule; Rat CD59 Overexpression Lysate; CD59 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
89aa
Sequence
Met1-Asn100
Applicable Applications for CD59 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Rat
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the rat CD59 (P27274) (Met1-Asn100) was expressed, fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CD59 cell lysate
CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
This Rat CD59 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD59 protein from the overexpression lysate was verified.
This Rat CD59 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD59 protein from the overexpression lysate was verified.
Product Categories/Family for CD59 cell lysate
References
Fletcher CM. et al., 1994, Structure. 2: 185-99. Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44. Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81. Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9. Picariello G. et al., 2008, Proteomics 8: 3833-47. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,790 Da
NCBI Official Full Name
CD59 glycoprotein
NCBI Official Synonym Full Names
CD59 molecule, complement regulatory protein
NCBI Official Symbol
Cd59
NCBI Official Synonym Symbols
Cd59a; Cd59b; MACIF; MACIP; MAC-IP
NCBI Protein Information
CD59 glycoprotein; CD59 antigen; CD59b moleucle, complement regulatory protein; Cb59b molecule; MAC-inhibitory protein; membrane attack complex inhibition factor; protectin
UniProt Protein Name
CD59 glycoprotein
Protein Family
UniProt Gene Name
Cd59
UniProt Synonym Gene Names
MAC-IP; MACIF
UniProt Entry Name
CD59_RAT
NCBI Description
may play a role in regulation of complement; may bind complement proteins C8 and C9 [RGD, Feb 2006]
Uniprot Description
CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Protein type: Membrane protein, GPI anchor
Cellular Component: anchored to external side of plasma membrane; extracellular space; compact myelin; cell surface; focal adhesion; plasma membrane; sarcolemma; vesicle
Molecular Function: complement binding
Biological Process: negative regulation of cytolysis; cell surface receptor linked signal transduction; negative regulation of complement activation; regulation of complement activation; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; negative regulation of apoptosis
Research Articles on CD59
1. In rats, CD59 and DAF genes are related to the negative effect of sulfasalazine on sperm maturation.
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Product Notes
The CD59 cd59 (Catalog #AAA8117534) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 89aa. AAA Biotech's CD59 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn10 0. It is sometimes possible for the material contained within the vial of "CD59, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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