Western Blot (WB)
(Anti-CD59 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat Spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa)
Rabbit anti-Rat CD59 Polyclonal Antibody | anti-CD59 antibody
Anti-CD59 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-5uL/mg of lysate
Western Blot (WB)
(Anti-CD59 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat Spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa)
Western Blot (WB)
(Anti-CD59 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat Spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa)
Immunoprecipitation (IP)
(ratCD59 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-ratCD59 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratCD59 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa Observed band size :17 kDa)
Immunoprecipitation (IP)
(ratCD59 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-ratCD59 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratCD59 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa Observed band size :17 kDa)
NCBI and Uniprot Product Information
NCBI Description
may play a role in regulation of complement; may bind complement proteins C8 and C9 [RGD, Feb 2006]
Uniprot Description
CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Protein type: Membrane protein, GPI anchor
Cellular Component: anchored to external side of plasma membrane; extracellular space; compact myelin; cell surface; focal adhesion; plasma membrane; sarcolemma; vesicle
Molecular Function: complement binding
Biological Process: negative regulation of cytolysis; cell surface receptor linked signal transduction; negative regulation of complement activation; regulation of complement activation; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; negative regulation of apoptosis
Research Articles on CD59
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Product Notes
The CD59 cd59 (Catalog #AAA8112915) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD59 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD59 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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