Peroxiredoxin 2 Cell Lysate | PRDX2 cell lysate
Mouse Peroxiredoxin 2 Baculovirus-Insect Overexpression Lysate
Gene Names
Prdx2; TR; PRP; TPx; TSA; TDX1; NkefB; PrxII; TPx-B; Tdpx1; Torin; Band-8; AL022839
Applications
Western Blot
Synonyms
Peroxiredoxin 2; Mouse Peroxiredoxin 2 Baculovirus-Insect Overexpression Lysate; Mouse Peroxiredoxin 2/PRDX2 Insect Cell Lysate (WB positive control); peroxiredoxin 2; Mouse AL022839 Overexpression Lysate; Mouse Band-8 Overexpression Lysate; Mouse NkefB Overexpression Lysate; Mouse PRP Overexpression Lysate; Mouse PrxII Overexpression Lysate; Mouse Tdpx1 Overexpression Lysate; Mouse TDX1 Overexpression Lysate; Mouse Torin Overexpression Lysate; Mouse TPx Overexpression Lysate; Mouse TPx-B Overexpression Lysate; Mouse TR Overexpression Lysate; Mouse TSA Overexpression Lysate; PRDX2 cell lysate
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
217aa
Sequence
Met1-Asn198
Applicable Applications for PRDX2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Sequence Construction
A DNA sequence encoding the mouse PRDX2 (NP_035693.3) (Met1-Asn198) was expressed with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PRDX2 cell lysate
Peroxiredoxin-2, also known as Natural killer cell-enhancing factor B, NKEF-B, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2 and NKEFB, is a cytoplasm protein that belongs to the ahpC/TSA family. Peroxiredoxin-2/PRDX2 contains one thioredoxin domain. Peroxiredoxin-2/PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-2/PRDX2 is not able to receive electrons from glutaredoxin. It may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-2/PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The Peroxiredoxins/Prx are a family of peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins/Prx family is divided into six groups (PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins/Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins/Prx has a proliferative effect and may be related to cancer development or progression.
This Mouse Peroxiredoxin 2 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of Peroxiredoxin 2 protein from the overexpression lysate was verified.
This Mouse Peroxiredoxin 2 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of Peroxiredoxin 2 protein from the overexpression lysate was verified.
References
Cha M.-K., et al., 1995, Biochem. Biophys. Res. Commun. 217:900-7. Noh, D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. Chevallet M., et al., 2003, J. Biol. Chem. 278:37146-53. Schremmer, B. et al., 2007, Subcell Biochem. 44 :317-44. Gauci S., et al., 2009, Anal. Chem. 81:4493-501.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,779 Da
NCBI Official Full Name
peroxiredoxin-2
NCBI Official Synonym Full Names
peroxiredoxin 2
NCBI Official Symbol
Prdx2
NCBI Official Synonym Symbols
TR; PRP; TPx; TSA; TDX1; NkefB; PrxII; TPx-B; Tdpx1; Torin; Band-8; AL022839
NCBI Protein Information
peroxiredoxin-2
UniProt Protein Name
Peroxiredoxin-2
Protein Family
UniProt Gene Name
Prdx2
UniProt Synonym Gene Names
Tdpx1; Tpx; TSA
Uniprot Description
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
Research Articles on PRDX2
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Product Notes
The PRDX2 prdx2 (Catalog #AAA8117144) is a Cell Lysate produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 217aa. AAA Biotech's Peroxiredoxin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PRDX2 prdx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn19 8. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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