IL3 cell lysate
Mouse IL3 HEK293 Overexpression Lysate
Gene Names
Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
Applications
Western Blot
Synonyms
IL3; Mouse IL3 HEK293 Overexpression Lysate; Mouse IL3/IL-3 HEK293 Cell Lysate (WB positive control); interleukin 3; Mouse BPA Overexpression Lysate; Mouse Csfmu Overexpression Lysate; Mouse HCGF Overexpression Lysate; Mouse Il-3 Overexpression Lysate; Mouse MCGF Overexpression Lysate; Mouse PSF Overexpression Lysate; IL3 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
140aa
Sequence
Met1-Cys166
Applicable Applications for IL3 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse IL3 (NP_034686.2) (Met1-Cys166) was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IL3 cell lysate
IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
This Mouse IL3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL3 protein from the overexpression lysate was verified.
This Mouse IL3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL3 protein from the overexpression lysate was verified.
References
Meyer CG, et al.. (2011) IL3 variant on chromosomal region 5q31-33 and protection from recurrent malaria attacks. Hum Mol Genet. 20(6):1173-81.Zambrano A, et al. (2010) IL-3 controls tau modifications and protects cortical neurons from neurodegeneration. Curr Alzheimer Res. 7(7):615-24.Dentelli P, et al.. (2011) IL-3 is a novel target to interfere with tumor vasculature. Oncogene. 30(50):4930-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,540 Da
NCBI Official Full Name
interleukin-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
Il3
NCBI Official Synonym Symbols
BPA; PSF; HCGF; Il-3; MCGF; Csfmu
NCBI Protein Information
interleukin-3
UniProt Protein Name
Interleukin-3
Protein Family
UniProt Gene Name
Il3
UniProt Synonym Gene Names
Csfmu; Il-3; IL-3; MCGF
UniProt Entry Name
IL3_MOUSE
Uniprot Description
IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.
Protein type: Apoptosis; Cell cycle regulation; Cytokine; Oncoprotein; Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Molecular Function: cytokine activity; growth factor activity; interleukin-3 receptor binding
Biological Process: activation of JAK protein; cytokine and chemokine mediated signaling pathway; hemopoiesis; monocyte differentiation; myeloid cell apoptosis; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of JNK cascade; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; regulation of cell growth; regulation of gene expression; regulation of glycolysis; regulation of protein amino acid dephosphorylation; response to hormone stimulus; response to hypoxia
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Product Notes
The IL3 il3 (Catalog #AAA8117016) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 140aa. AAA Biotech's IL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IL3 il3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Cys16 6. It is sometimes possible for the material contained within the vial of "IL3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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