Mouse IL-3 ELISA Kit | IL3 elisa kit

Mouse IL-3 PicoKine ELISA Kit

Cat. Num. AAA175944

• Gene Names: Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Reactivity: Mouse
• Synonyms: IL-3; Mouse IL-3 PicoKine ELISA Kit; Interleukin-3; interleukin 3; Burst promoting activity; Colony stimulating factor multiple; Eosinophil colony stimulating factor; Hematopoietic growth factor; IL 3; IL3; IL3_HUMAN; Interleukin 3; Mast cell growth factor; MCGF; Multi CSF; Multilineage colony stimulating factor; Multipotential colony stimulating factor; Multipotential colony-stimulating factor; P cell stimulating factor; P-cell-stimulating factor; Thy1 inducing factor; IL3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IL-3
• Sequence Length: 166

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse IL-3 PicoKine ELISA Kit standard curve )

Related Product Information for IL3 elisa kit

Principle of the assay: mouse IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-3 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-3 amount of sample captured in plate.
Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, ischemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors thatregulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 Themouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the longarm of chromosome 5.5 The standard product used in this kit is recombinant mouse IL-3, consisting of 135 aminoacids with the molecular mass of 15KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 124333
• NCBI GeneID: 16187
• UniProt Accession #: P01586
• Molecular Weight: 18,540 Da
• NCBI Official Full Name: Interleukin-3
• NCBI Official Synonym Full Names: interleukin 3
• NCBI Official Symbol: Il3
• NCBI Official Synonym Symbols: BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• NCBI Protein Information: interleukin-3; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: Il3
• UniProt Synonym Gene Names: Csfmu; Il-3; IL-3; MCGF
• UniProt Entry Name: IL3_MOUSE

Uniprot Description

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Cell cycle regulation; Cytokine; Apoptosis; Secreted, signal peptide; Secreted; Oncoprotein

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity

Biological Process: myeloid cell apoptosis; regulation of protein amino acid dephosphorylation; negative regulation of autophagy; cytokine and chemokine mediated signaling pathway; response to hormone stimulus; positive regulation of JNK cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of gene expression; negative regulation of mast cell apoptosis; regulation of glycolysis; positive regulation of cell proliferation; response to hypoxia; hemopoiesis; immune response; regulation of cell growth; positive regulation of protein amino acid phosphorylation; positive regulation of myeloid leukocyte differentiation; negative regulation of apoptosis