BAMBI cell lysate
Mouse BAMBI HEK293 Overexpression Lysate
Gene Names
Bambi; 2610003H06Rik
Applications
Western Blot
Synonyms
BAMBI; Mouse BAMBI HEK293 Overexpression Lysate; Mouse BAMBI/NMA HEK293 Cell Lysate (WB positive control); BMP and activin membrane-bound inhibitor; Mouse 2610003H06Rik Overexpression Lysate; BAMBI cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
367aa
Sequence
Met1-Ala152
Applicable Applications for BAMBI cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse BAMBI (Q9D0L6) (Met1-Ala152) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for BAMBI cell lysate
BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/ threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor-beta (TGF-beta), through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta-catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.
This Mouse BAMBI overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of BAMBI protein from the overexpression lysate was verified.
This Mouse BAMBI overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of BAMBI protein from the overexpression lysate was verified.
References
Sekiya T, et al. (2003) Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor-Signaling, as a Target of the -Catenin Pathway in Colorectal Tumor Cells. The Journal of Biological Chemistry. 279:6840-6. Shi YG, et al. (2003) Mechanisms of TGF-Signaling from Cell Membrane to the Nucleus. Cell. 113(6): 685-700.Wanninger J, et al. (2011) Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett. 585(9):1338-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,198 Da
NCBI Official Full Name
BMP and activin membrane-bound inhibitor homolog
NCBI Official Synonym Full Names
BMP and activin membrane-bound inhibitor
NCBI Official Symbol
Bambi
NCBI Official Synonym Symbols
2610003H06Rik
NCBI Protein Information
BMP and activin membrane-bound inhibitor homolog
UniProt Protein Name
BMP and activin membrane-bound inhibitor homolog
Protein Family
UniProt Gene Name
Bambi
UniProt Synonym Gene Names
Nma
Uniprot Description
BAMBI: Negatively regulates TGF-beta signaling. Belongs to the BAMBI family.
Protein type: Inhibitor; Membrane protein, integral
Chromosomal Location of Human Ortholog: 18|18 A1
Cellular Component: cytoplasm; integral component of membrane; membrane; plasma membrane
Molecular Function: frizzled binding
Biological Process: cell migration; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of protein binding; positive regulation of transcription, DNA-templated; regulation of cell shape; transforming growth factor beta receptor signaling pathway
Research Articles on BAMBI
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Product Notes
The BAMBI bambi (Catalog #AAA8116918) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 367aa. AAA Biotech's BAMBI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the BAMBI bambi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ala15 2. It is sometimes possible for the material contained within the vial of "BAMBI, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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