LYPD3 cell lysate
Mouse LYPD3 HEK293 Overexpression Lysate
Gene Names
Lypd3; C4.4a; 2310061G07Rik
Applications
Western Blot
Synonyms
LYPD3; Mouse LYPD3 HEK293 Overexpression Lysate; Mouse C4.4A/LYPD3 HEK293 Cell Lysate (WB positive control); LY6/PLAUR domain containing 3; Mouse 2310061G07Rik Overexpression Lysate; Mouse C4.4a Overexpression Lysate; LYPD3 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
266aa
Sequence
Met1-His287
Applicable Applications for LYPD3 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the N-terminal segment of mouse LYPD3 (NP_598504.1) (Met 1-His 287) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for LYPD3 cell lysate
Ly6/PLAUR domain-containing protein 3, also known as GPI-anchored metastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein, MIG-C4, and LYPD3, is a cell membrane protein that contains two UPAR/Ly6 domains. Human LYPD3 contains two UPAR/Ly6 domains. LYPD3 is expressed in the placenta, skin, and urothelium. It is found in suprabasal keratinocytes of chronic wounds. Weak expression of LYPD3 is found in the esophagus and peripheral blood mononuclear cells. It is found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression of LYPD3 is found in the tumor component of some noninvasive superficial lesions and invasive and metastatic urothelial cancers. LYPD3 is up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds. LYPD3 supports cell migration. It may be involved in urothelial cell-matrix interactions. It may also be involved in tumor progression
This Mouse LYPD3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LYPD3 protein from the overexpression lysate was verified.
This Mouse LYPD3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LYPD3 protein from the overexpression lysate was verified.
References
Smith B.A., et al., 2001, Cancer Res. 61:1678-85. Wuerfel J., et al., 2001, Gene 262:35-41. Clark H.F., et al., 2003, Genome Res. 13:2265-70. Fletcher G.C., et al., 2003, Br. J. Cancer 88:579-85. Hansen L.V., et al., 2004, Biochem. Eng. J. 380:845-57.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,489 Da
NCBI Official Full Name
ly6/PLAUR domain-containing protein 3
NCBI Official Synonym Full Names
Ly6/Plaur domain containing 3
NCBI Official Symbol
Lypd3
NCBI Official Synonym Symbols
C4.4a; 2310061G07Rik
NCBI Protein Information
ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein homolog; GPI-anchored metastasis-associated protein C4.4A homolog
UniProt Protein Name
Ly6/PLAUR domain-containing protein 3
Protein Family
UniProt Gene Name
Lypd3
UniProt Synonym Gene Names
C4.4a
UniProt Entry Name
LYPD3_MOUSE
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Product Notes
The LYPD3 lypd3 (Catalog #AAA8116687) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 266aa. AAA Biotech's LYPD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the LYPD3 lypd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-His28 7. It is sometimes possible for the material contained within the vial of "LYPD3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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