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SDS-Page (SDS-PAGE)

Colony Stimulating Factor 1, Macrophage (MCSF) Recombinant Protein | MCSF recombinant protein

Recombinant Colony Stimulating Factor 1, Macrophage (MCSF)

Gene Names
CSF1; MCSF; CSF-1
Applications
SDS-Page, Western Blot
Purity
Greater than 95% by SDS-PAGE
Synonyms
Colony Stimulating Factor 1; Macrophage (MCSF); Recombinant Colony Stimulating Factor 1; MCSF/Colony Stimulating Factor 1; Macrophage; CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1; MCSF recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence
Glu33~Asn190
Applicable Applications for MCSF recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Tag
N-terminal His Tag
Expression System
Prokaryotic expression
Organism Species
Human
Isoelectric Point
4.6
Subcellular Location
Membrane
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

SDS-Page

(SDS-PAGE)

SDS-Page (SDS-PAGE)
Product Categories/Family for MCSF recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
60,179 Da
NCBI Official Full Name
colony-stimulating factor (CSF-1)
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
CSF1
NCBI Official Synonym Symbols
MCSF; CSF-1
NCBI Protein Information
macrophage colony-stimulating factor 1; lanimostim
UniProt Protein Name
Macrophage colony-stimulating factor 1
UniProt Gene Name
CSF1
UniProt Synonym Gene Names
CSF-1; M-CSF; MCSF
UniProt Entry Name
CSF1_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; receptor complex

Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; monocyte activation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; hemopoiesis; positive regulation of Ras protein signal transduction; positive regulation of macrophage differentiation; cell differentiation; positive regulation of cell-matrix adhesion; inflammatory response; regulation of ossification; positive regulation of cell migration

Research Articles on MCSF

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Product Notes

The MCSF csf1 (Catalog #AAA2125409) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Colony Stimulating Factor 1, Macrophage (MCSF) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the MCSF csf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu33~Asn1 90. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 1, Macrophage (MCSF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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