B3GNT6 cell lysate
Human B3GNT6 Baculovirus-Insect cells Overexpression Lysate
Gene Names
B3GNT6; BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
Applications
Western Blot
Synonyms
B3GNT6; Human B3GNT6 Baculovirus-Insect cells Overexpression Lysate; Human B3GNT6 (aa 44-384) Insect Cell Lysate (WB positive control); UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 6; Human B3Gn-T6 Overexpression Lysate; Human beta-1; 3-Gn-T6 Overexpression Lysate; Human beta3Gn-T6 Overexpression Lysate; Human BGnT-6 Overexpression Lysate; B3GNT6 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
357aa
Sequence
Gln44-Ser384
Applicable Applications for B3GNT6 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human B3GNT6 (NP_619651.3) (Gln44-Ser384) was fused with a polyhistide tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for B3GNT6 cell lysate
B3GNT6 belongs to the glycosyltransferase 31 family. B3GNT6 play s an important role in the synthesis of mucin-type O-glycans in digestive organs. It catalyzes the transfer of GlcNAc from UDP-GlcNAc to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-Ser/Thr). Core 3 structure exists in O-glycan which is an important precursor in the biosynthesis of mucin-type glycoproteins. Loss of core 3 could lead to the production of secreted mucins, then bacteria would be inefficiently cleared from the system, and chronic inflammation would be developed, which eventually would result in development of cancer. B3GNT6 gene is a tumor suppressor gene.
This Human B3GNT6 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of B3GNT6 protein from the overexpression lysate was verified.
This Human B3GNT6 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of B3GNT6 protein from the overexpression lysate was verified.
References
Hennet T, et al. (1998) Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1, 3-galactosyltransferase genes. J Biol Chem. 273(1):58-65. Kolbinger F, et al. (1998) Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains. J Biol Chem. 273(1): 433-40. Henrissat B, et al. (1997) A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem J. 326:929-39.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,748 Da
NCBI Official Full Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
NCBI Official Symbol
B3GNT6
NCBI Official Synonym Symbols
BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
NCBI Protein Information
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6; core 3 synthase; beta-1,3-N-acetylglucosaminyltransferase protein
UniProt Protein Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
Protein Family
UniProt Gene Name
B3GNT6
UniProt Synonym Gene Names
BGnT-6; Beta-1,3-Gn-T6; Beta-1,3-N-acetylglucosaminyltransferase 6; Beta3Gn-T6
UniProt Entry Name
B3GN6_HUMAN
Uniprot Description
B3GNT6: Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.-
Chromosomal Location of Human Ortholog: 11q13.4
Cellular Component: Golgi membrane; membrane; integral to membrane
Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein biosynthetic process; O-glycan processing; O-glycan processing, core 3; post-translational protein modification
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Product Notes
The B3GNT6 b3gnt6 (Catalog #AAA8115319) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 357aa. AAA Biotech's B3GNT6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the B3GNT6 b3gnt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gln44-Ser3 84. It is sometimes possible for the material contained within the vial of "B3GNT6, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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