RP2 cell lysate

Human RP2 Baculovirus-Insect cells Overexpression Lysate

Gene Names

RP2; XRP2; NME10; TBCCD2; NM23-H10; DELXp11.3

Applications

Western Blot

Synonyms

RP2; Human RP2 Baculovirus-Insect cells Overexpression Lysate; Human XRP2/RP2 Insect Cell Lysate (WB positive control); retinitis pigmentosa 2 (X-linked recessive); Human DELXp11.3 Overexpression Lysate; Human KIAA0215 Overexpression Lysate; Human NM23-H10 Overexpression Lysate; Human NME10 Overexpression Lysate; Human RP2 Overexpression Lysate; Human TBCCD2 Overexpression Lysate; Human XRP2 Overexpression Lysate; RP2 cell lysate

Ordering
Cat. Num. AAA8114421 For Research Use Only!

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Sequence Positions

575aa

Sequence

Met1-Thr350

Applicable Applications for RP2 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human XRP2 (NP_008846.2) (Met 1-Thr 350) was fused with the GST tag at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Related Product Information for RP2 cell lysate

XRP2, also known as Protein XRP2 and RP2, is a member of the TBCC (tubulin cofactor C) family and contains one C-CAP/cofactor C-like domain. This protein is encoded by the RP2 gene in humans. XRP2 stimulates the GTPase activity of tubulin, but does not enhance tubulin heterodimerization. XRP2 acts as guanine nucleotide dissociation inhibitor for ARL3. Defects in RP2 gene are the cause of retinitis pigmentosa type 2 (RP2), also known as X-linked retinitis pigmentosa 2 (XLRP-2). It leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

This Human RP2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of RP2 protein from the overexpression lysate was verified.

References

Schwahn U., et al., (1998), Positional cloning of the gene for X-linked retinitis pigmentosa 2. Nat. Genet. 19:327-332.Ross M.T., et al., (2005), The DNA sequence of the human X chromosome.Nature 434:325-337.Vaca Jacome A.S., et al., (2015), N-terminome analysis of the human mitochondrial proteome. Proteomics 15:2519-2524.

NCBI and Uniprot Product Information

NCBI GI #

170016081

NCBI GeneID

6102

NCBI Accession #

NP_008846.2

NCBI GenBank Nucleotide #

NM_006915.2

UniProt Accession #

O75695; Q86XJ7; Q9NU67

Molecular Weight

39,641 Da

NCBI Official Full Name

protein XRP2

NCBI Official Synonym Full Names

RP2, ARL3 GTPase activating protein

NCBI Official Symbol

RP2

NCBI Official Synonym Symbols

XRP2; NME10; TBCCD2; NM23-H10; DELXp11.3

NCBI Protein Information

protein XRP2

UniProt Protein Name

Protein XRP2

Protein Family

Protein

UniProt Gene Name

RP2

UniProt Entry Name

XRP2_HUMAN

NCBI Description

The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly-folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death [provided by RefSeq, Jul 2008]

Uniprot Description

RP2: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. Defects in RP2 are the cause of retinitis pigmentosa type 2 (RP2); also known as X-linked retinitis pigmentosa 2 (XLRP-2). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the TBCC family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: centriole; Golgi apparatus; cytoplasm; plasma membrane; cytoplasmic vesicle

Molecular Function: protein binding; GTP binding; unfolded protein binding; nucleoside diphosphate kinase activity; actin binding; GTPase activator activity; ATP binding

Biological Process: GTP biosynthetic process; CTP biosynthetic process; protein transport; protein folding; visual perception; organelle organization and biogenesis; UTP biosynthetic process; cell morphogenesis; nucleoside diphosphate phosphorylation; cytoskeleton organization and biogenesis; post-Golgi vesicle-mediated transport; post-chaperonin tubulin folding pathway; positive regulation of GTPase activity

Disease: Retinitis Pigmentosa 2

Research Articles on RP2

1. RP2 mutation would have a moderate pathogenic effect in photoreceptors carrying the mutation, causing abnormal outer segments, with the accumulation of lipofuscin similar to RDS/PRPH2 pattern dystrophy.

Similar Products

RP2 Recombinant Protein

Host: Baculovirus-Insect Cells

Reactivity: n/a

Apps: n/a

XRP2 / RP2 Recombinant Protein

Host: Baculovirus-Insect Cells

Reactivity: n/a

Apps: n/a

RP2 Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell

Reactivity: n/a

Apps: n/a

RP2 Antibody

Host: Rabbit

Reactivity: Human

Apps: ELISA, Immunohistochemistry

RP2 Antibody

Host: Mouse

Reactivity: n/a

Apps: Immunofluorescence, Western Blot

RP2 Antibody

Host: Mouse

Reactivity: n/a

Apps: Western Blot

RP2 Antibody

Host: Mouse

Reactivity: n/a

Apps: Immunofluorescence, Western Blot

RP2 Antibody

Host: Mouse

Reactivity: n/a

Apps: Immunofluorescence, Western Blot

RP2 Antibody

Host: Mouse

Reactivity: n/a

Apps: Immunofluorescence, Western Blot

RP2 Antibody

Host: Rabbit

Reactivity: Human

Apps: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation

RP2 Antibody

Host: Rabbit

Reactivity: Human, Mouse, Rat

Apps: Western Blot

Product Notes
The RP2 rp2 (Catalog #AAA8114421) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 575aa. AAA Biotech's RP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the RP2 rp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr35 0. It is sometimes possible for the material contained within the vial of "RP2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.