Rabbit anti-Human RP2 Polyclonal Antibody | anti-RP2 antibody

RP2 Antibody

Cat. Num. AAA7044960

• Gene Names: RP2; XRP2; NME10; TBCCD2; NM23-H10; DELXp11.3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: RP2; Polyclonal Antibody; RP2 Antibody; Protein XRP2; anti-RP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 350

• Applicable Applications for anti-RP2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Species: Human
• Immunogen: Recombinant human Protein XRP2 protein (1-350AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79710
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes:  RP2antibody at 3.81ug/mlLane 1 : Jurkat whole cell lysateLane 2 : HepG-2 whole cell lysateLane 3 : Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 40 kDaObserved band size: 40 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7044960 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating RP2 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug) instead of MBS7044960 in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: MBS7044960(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))

Related Product Information for anti-RP2 antibody

Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 170016081
• NCBI GeneID: 6102
• NCBI Accession #: NP_008846.2
• NCBI GenBank Nucleotide #: NM_006915.2
• UniProt Accession #: O75695; Q86XJ7; Q9NU67
• Molecular Weight: 39,641 Da
• NCBI Official Full Name: protein XRP2
• NCBI Official Synonym Full Names: retinitis pigmentosa 2 (X-linked recessive)
• NCBI Official Symbol: RP2
• NCBI Official Synonym Symbols: XRP2; NME10; TBCCD2; NM23-H10; DELXp11.3
• NCBI Protein Information: protein XRP2
• UniProt Protein Name: Protein XRP2
• Protein Family: Protein
• UniProt Gene Name: RP2
• UniProt Entry Name: XRP2_HUMAN

NCBI Description

The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly-folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death [provided by RefSeq, Jul 2008]

Uniprot Description

RP2: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. Defects in RP2 are the cause of retinitis pigmentosa type 2 (RP2); also known as X-linked retinitis pigmentosa 2 (XLRP-2). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the TBCC family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: cytoplasm; cytoplasmic vesicle; plasma membrane

Molecular Function: GTPase activator activity; protein binding; unfolded protein binding

Biological Process: post-chaperonin tubulin folding pathway; post-Golgi vesicle-mediated transport; protein folding; visual perception

Disease: Retinitis Pigmentosa 2