Rabbit anti-Mouse Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Anti-Tyrosine Hydroxylase Antibody, Rabbit Polyclonal

Cat. Num. AAA8112224

• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; Anti-Tyrosine Hydroxylase Antibody; Rabbit Polyclonal; TH/Tyrosine Hydroxylase Antibody; Rabbit PAb; tyrosine hydroxylase; anti-TH antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse TH/Tyrosine Hydroxylase
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TH antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Mouse TH/Tyrosine Hydroxylase protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse TH/Tyrosine Hydroxylase. Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-TH antibody

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state, and the protein that transfers dopamine into secretory vesicles. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Product Categories/Family for anti-TH antibody

Neurotransmitter Associated Enzymes; G Protein-coupled Receptors Signaling

References

Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.

NCBI and Uniprot Product Information

• NCBI GI #: 6678337
• NCBI GeneID: 21823
• NCBI Accession #: NP_033403.1
• NCBI GenBank Nucleotide #: NM_009377.1
• UniProt Accession #: P24529
• Molecular Weight: 55,993 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: Th
• NCBI Protein Information: tyrosine 3-monooxygenase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: Th
• UniProt Synonym Gene Names: TH

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Endoplasmic reticulum; Mitochondrial; Oxidoreductase; Vesicle

Chromosomal Location of Human Ortholog: 7 F5|7 88.06 cM

Cellular Component: axon; cell soma; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; cytosol; dendrite; internal side of plasma membrane; melanosome membrane; mitochondrion; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum; synaptic vesicle; terminal button

Molecular Function: amino acid binding; dopamine binding; enzyme binding; ferric iron binding; ferrous iron binding; monooxygenase activity; oxygen binding; protein domain specific binding; tyrosine 3-monooxygenase activity

Biological Process: catecholamine biosynthetic process; dopamine biosynthetic process; dopamine biosynthetic process from tyrosine; eating behavior; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; heart development; learning; locomotory behavior; mating behavior; memory; norepinephrine biosynthetic process; organ morphogenesis; regulation of heart contraction; response to ethanol; response to hypoxia; synaptic transmission, dopaminergic; synaptic vesicle amine transport; visual perception