Tyrosine Hydroxylase Recombinant Protein | TH recombinant protein

Tyrosine Hydroxylase Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123082

• Purity: >85% as determined by SDS-PAGE
• Synonyms: Tyrosine Hydroxylase; Tyrosine Hydroxylase Protein; Mouse; Recombinant (His Tag); Mouse TH/Tyrosine Hydroxylase Protein (His Tag); tyrosine hydroxylase; TH recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro2-Ser498

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse TH (P24529)(Pro 2-Ser 498) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TH recombinant protein

Background: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state, and the protein that transfers dopamine into secretory vesicles. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

Product Categories/Family for TH recombinant protein

Neurotransmitter Associated Enzymes; G Protein-coupled Receptors Signaling

References

Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.

NCBI and Uniprot Product Information

• NCBI GI #: 6678337
• NCBI GeneID: 21823
• NCBI Accession #: NP_033403.1
• NCBI GenBank Nucleotide #: NM_009377.1
• UniProt Accession #: P24529
• Molecular Weight: 55,993 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: Th
• NCBI Protein Information: tyrosine 3-monooxygenase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: Th
• UniProt Synonym Gene Names: TH

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Endoplasmic reticulum; Mitochondrial; Oxidoreductase; Vesicle

Chromosomal Location of Human Ortholog: 7 F5|7 88.06 cM

Cellular Component: axon; cell soma; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; cytosol; dendrite; internal side of plasma membrane; melanosome membrane; mitochondrion; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum; synaptic vesicle; terminal button

Molecular Function: amino acid binding; dopamine binding; enzyme binding; ferric iron binding; ferrous iron binding; monooxygenase activity; oxygen binding; protein domain specific binding; tyrosine 3-monooxygenase activity

Biological Process: catecholamine biosynthetic process; dopamine biosynthetic process; dopamine biosynthetic process from tyrosine; eating behavior; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; heart development; learning; locomotory behavior; mating behavior; memory; norepinephrine biosynthetic process; organ morphogenesis; regulation of heart contraction; response to ethanol; response to hypoxia; synaptic transmission, dopaminergic; synaptic vesicle amine transport; visual perception