Immunohistochemistry (IHC)
(Immunochemical staining of mouse CAMK4 in mouse brain with rabbit polyclonal antibody at 1:50000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Mouse CAMKIV Polyclonal Antibody | anti-CAMK4 antibody
Anti-CAMKIV Antibody, Rabbit Polyclonal
ELISA: 1:25,000-1:50,000
IHC-P: 1:5000-1:50,000
IP: 0.2-1uL/mg of lysate
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CAMK4 in mouse brain with rabbit polyclonal antibody at 1:50000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CAMK4 in mouse brain with rabbit polyclonal antibody at 1:50000 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(Mouse CAMK4 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-Mouse CAMK4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CAMK4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
Immunoprecipitation (IP)
(Mouse CAMK4 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-Mouse CAMK4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CAMK4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size: 58 kDa)
Western Blot (WB)
(Anti-CAMK4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CAMK4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa (We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
Uniprot Description
CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.
Protein type: EC 2.7.11.17; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; CAMK1 family
Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: transferase activity; calmodulin binding; protein serine/threonine kinase activity; protein binding; calcium-dependent protein serine/threonine kinase activity; calmodulin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: immune system process; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; nucleocytoplasmic transport; myeloid dendritic cell differentiation; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; peptidyl-serine phosphorylation; long-term memory; nerve-nerve synaptic transmission; inflammatory response; phosphorylation
Research Articles on CAMK4
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Product Notes
The CAMK4 camk4 (Catalog #AAA8112125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CAMKIV Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAMKIV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:5000-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the CAMK4 camk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMKIV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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