Testing Data
(Sample(30 ug whole cell lysate)A: A431 7.5% SDS PAGEPrimary antibody diluted at 1: 5000)
Rabbit anti-Human SART1 Polyclonal Antibody | anti-SART1 antibody
SART1 antibody
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Testing Data
(Sample(30 ug whole cell lysate)A: A431 7.5% SDS PAGEPrimary antibody diluted at 1: 5000)
Testing Data
(Sample(30 ug whole cell lysate)A: A431 7.5% SDS PAGEPrimary antibody diluted at 1: 5000)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SART1 antibody at 1: 500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SART1 antibody at 1: 500 dilution.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using SART1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using SART1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]
Uniprot Description
SART1: May play a role in mRNA splicing. May also bind to DNA. Belongs to the SNU66/SART1 family. 1 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; RNA-binding; RNA processing; Spliceosome; RNA splicing
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: nucleoplasm; Golgi apparatus; Cajal body; U4/U6 x U5 tri-snRNP complex; cytoplasm; cytosol
Molecular Function: protein binding
Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; positive regulation of cytotoxic T cell differentiation; maturation of 5S rRNA; nuclear mRNA cis splicing, via U2-type spliceosome; cell cycle arrest
Research Articles on SART1
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Product Notes
The SART1 sart1 (Catalog #AAA9404816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SART1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SART1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:500 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the SART1 sart1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SART1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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