Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLK Polyclonal Antibody )
Rabbit POLK Polyclonal Antibody | anti-POLK antibody
POLK Polyclonal Antibody
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLK Polyclonal Antibody )
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLK Polyclonal Antibody )
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using POLK Polyclonal Antibody )
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using POLK Polyclonal Antibody )
NCBI and Uniprot Product Information
NCBI Description
External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010]
Uniprot Description
POLK: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Belongs to the DNA polymerase type-Y family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Transferase; EC 2.7.7.7; DNA replication
Chromosomal Location of Human Ortholog: 5q13
Cellular Component: nucleoplasm
Molecular Function: metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity
Biological Process: nucleotide-excision repair, DNA gap filling; DNA repair; DNA replication
Research Articles on POLK
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Product Notes
The POLK polk (Catalog #AAA2566557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLK Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLK can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:50-1:200. Researchers should empirically determine the suitability of the POLK polk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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