Rabbit anti-Mouse Prolactin Receptor Polyclonal Antibody | anti-PRLR antibody

Anti-Prolactin Receptor Antibody, Rabbit Polyclonal

Cat. Num. AAA8111600

• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Prolactin Receptor; Polyclonal Antibody; Anti-Prolactin Receptor Antibody; Rabbit Polyclonal; Prolactin Receptor/PRLR Antibody; Rabbit PAb; Antigen Affinity Purified; prolactin receptor; Anti-AI987712 Antibody; Anti-Pr-1 Antibody; Anti-Pr-3 Antibody; Anti-Prlr Antibody; Anti-Prlr-rs1 Antibody; anti-PRLR antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Prolactin Receptor/PRLR
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PRLR antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Mouse Prolactin Receptor/PRLR Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Prolactin Receptor/PRLR (rM Prolactin Receptor/PRLR). Prolactin Receptor/PRLR specific IgG was purified by Mouse Prolactin Receptor/PRLR affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-PRLR antibody

Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type cytokine receptor superfamily, and contains two fibronectin type-a domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules involved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

References

Bole-Feysot C, et al. (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev. 19(3): 225-68.Goffin V, et al. (1999) From the molecular biology of prolactin and its receptor to the lessons learned from knockout mice models. Genet Anal. 15(3-5): 189-201.Li Y, et al. (2006) Stabilization of prolactin receptor in breast cancer cells. Oncogene. 25(13): 1896-902.Shao R, et al. (2008) Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice. Biol Reprod. 79(4): 748-57.

NCBI and Uniprot Product Information

• NCBI GI #: 119528
• NCBI GeneID: 13856
• UniProt Accession #: P07321
• Molecular Weight: 21,365 Da
• NCBI Official Full Name: Erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Prolactin receptor
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_MOUSE

NCBI Description

This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; heart morphogenesis; erythrocyte maturation; apoptosis; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; negative regulation of myeloid cell apoptosis; peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of protein kinase activity; positive regulation of cell proliferation; cardiac muscle morphogensis; response to hypoxia; erythrocyte differentiation; ventricular cardiac muscle morphogenesis; angiogenesis; positive regulation of Ras protein signal transduction; positive regulation of neuron differentiation; embryo implantation; positive regulation of DNA replication; negative regulation of apoptosis