Rabbit anti-Human Prolactin Receptor Polyclonal Antibody | anti-PRLR antibody

Anti-Prolactin Receptor Antibody, Rabbit Polyclonal

Cat. Num. AAA8102084

• Gene Names: PRLR; hPRLrI
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Prolactin Receptor; Polyclonal Antibody; Anti-Prolactin Receptor Antibody; Rabbit Polyclonal; Prolactin Receptor/PRLR Antibody; Rabbit PAb; Antigen Affinity Purified; prolactin receptor; Anti-HPRL Antibody; Anti-hPRLrI Antibody; Anti-MFAB Antibody; Anti-PRLR Antibody; anti-PRLR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Prolactin Receptor/PRLR
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PRLR antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: Recombinant Human Prolactin Receptor/PRLR Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Prolactin Receptor/PRLR (rh Prolactin Receptor/PRLR). Prolactin Receptor/PRLR specific IgG was purified by Human Prolactin Receptor/PRLR affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PRLR in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human PRLR in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PRLR antibody

Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type cytokine receptor superfamily, and contains two fibronectin type-a domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules involved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

References

Bole-Feysot C, et al. (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev. 19(3): 225-68.Goffin V, et al. (1999) From the molecular biology of prolactin and its receptor to the lessons learned from knockout mice models. Genet Anal. 15(3-5): 189-201.Li Y, et al. (2006) Stabilization of prolactin receptor in breast cancer cells. Oncogene. 25(13): 1896-902.Shao R, et al. (2008) Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice. Biol Reprod. 79(4): 748-57.

NCBI and Uniprot Product Information

• NCBI GI #: 4506107
• NCBI GeneID: 5618
• NCBI Accession #: NP_000940.1
• NCBI GenBank Nucleotide #: NM_000949.5
• UniProt Accession #: P16471; Q16354; Q8TD75; Q8TD78; Q96P35; Q96P36; Q9BX87; Q9UHJ5; B2R882
• Molecular Weight: 24,000
• NCBI Official Full Name: prolactin receptor isoform 1
• NCBI Official Synonym Full Names: prolactin receptor
• NCBI Official Symbol: PRLR
• NCBI Official Synonym Symbols: hPRLrI
• NCBI Protein Information: prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein
• UniProt Protein Name: Prolactin receptor
• Protein Family: Prolactin receptor
• UniProt Gene Name: PRLR
• UniProt Synonym Gene Names: PRL-R
• UniProt Entry Name: PRLR_HUMAN

NCBI Description

This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011]

Uniprot Description

PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13.2

Cellular Component: cell surface; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; protein homodimerization activity; ornithine decarboxylase activator activity; peptide hormone binding; metal ion binding; prolactin receptor activity

Biological Process: regulation of cell adhesion; lactation; transmembrane receptor protein tyrosine kinase activation (dimerization); cell surface receptor linked signal transduction; T cell activation; regulation of epithelial cell differentiation; tyrosine phosphorylation of JAK2 protein; steroid biosynthetic process; embryo implantation; negative regulation of apoptosis

Disease: Multiple Fibroadenomas Of The Breast; Hyperprolactinemia