Prolactin Receptor Recombinant Protein | PRLR recombinant protein

Prolactin Receptor Protein, Mouse, Recombinant (His & hFc Tag)

Cat. Num. AAA8122750

• Gene Names: Prlr; Pr-1; Pr-3; AI987712; Prlr-rs1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Prolactin Receptor; Prolactin Receptor Protein; Mouse; Recombinant (His & hFc Tag); Mouse PRLR/Prolactin Receptor Protein (His & Fc Tag); AI987712 Protein; Pr-1 Protein; Pr-3 Protein; Prlr Protein; Prlr-rs1 Protein; prolactin receptor; PRLR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp229

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 21
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse PRLR (NP_035299.4)(Met 1-Asp 229) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PRLR recombinant protein

Background: Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type a- cytokine receptor superfamily, and contains two fibronectin type-a- c domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules involved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

References

Bole-Feysot C, et al. (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev. 19(3): 225-68.Goffin V, et al. (1999) From the molecular biology of prolactin and its receptor to the lessons learned from knockout mice models. Genet Anal. 15(3-5): 189-201.Li Y, et al. (2006) Stabilization of prolactin receptor in breast cancer cells. Oncogene. 25(13): 1896-902.Shao R, et al. (2008) Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice. Biol Reprod. 79(4): 748-57.

NCBI and Uniprot Product Information

• NCBI GI #: 254675324
• NCBI GeneID: 19116
• NCBI Accession #: NP_035299.4
• NCBI GenBank Nucleotide #: NM_011169.5
• UniProt Accession #: Q08501; P15212; P15213; Q62099
• Molecular Weight: 33,633 Da
• NCBI Official Full Name: prolactin receptor isoform 1
• NCBI Official Synonym Full Names: prolactin receptor
• NCBI Official Symbol: Prlr
• NCBI Official Synonym Symbols: Pr-1; Pr-3; AI987712; Prlr-rs1
• NCBI Protein Information: prolactin receptor
• UniProt Protein Name: Prolactin receptor
• Protein Family: Prolactin receptor
• UniProt Gene Name: Prlr
• UniProt Synonym Gene Names: PRL-R
• UniProt Entry Name: PRLR_MOUSE

Uniprot Description

PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; intracellular

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; peptide hormone binding; metal ion binding; protein kinase binding; prolactin receptor activity

Biological Process: regulation of cell adhesion; lactation; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of epithelial cell differentiation; JAK-STAT cascade