PRLR / Prolactin Receptor Recombinant Protein | PRLR recombinant protein

Recombinant Mouse PRLR / Prolactin Receptor Protein (His & Fc tag)

Cat. Num. AAA2546975

• Gene Names: Prlr; Pr-1; Pr-3; AI987712; Prlr-rs1
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: PRLR / Prolactin Receptor; Recombinant Mouse PRLR / Prolactin Receptor Protein (His & Fc tag); AI987712; Pr-1; Pr-3; Prlr-rs1; Prlr; PRLR recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 608

• Application Notes: The recombinant mouse PRLR/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 457 amino acids and has a calculated molecular mass of 52.5 kDa. As a result of glycosylation, the apparent molecular mass of the mouse PRLR/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PRLR recombinant protein

Background: Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type I  cytokine receptor superfamily, and contains two fibronectin type -I¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

Description: A DNA sequence encoding the extracellular domain of mouse PRLR (NP_035299.4) (Met 1-Asp 229) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 254675324
• NCBI GeneID: 19116
• NCBI Accession #: NP_035299.4
• NCBI GenBank Nucleotide #: NM_011169.5
• UniProt Accession #: Q08501; P15212; P15213; Q62099
• Molecular Weight: 33,633 Da
• NCBI Official Full Name: prolactin receptor isoform 1
• NCBI Official Synonym Full Names: prolactin receptor
• NCBI Official Symbol: Prlr
• NCBI Official Synonym Symbols: Pr-1; Pr-3; AI987712; Prlr-rs1
• NCBI Protein Information: prolactin receptor
• UniProt Protein Name: Prolactin receptor
• Protein Family: Prolactin receptor
• UniProt Gene Name: Prlr
• UniProt Synonym Gene Names: PRL-R

Uniprot Description

PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 15 A1|15 5.23 cM

Cellular Component: cell surface

Molecular Function: peptide hormone binding; prolactin receptor activity; protein binding; protein kinase binding

Biological Process: JAK-STAT cascade; lactation; regulation of cell adhesion; regulation of epithelial cell differentiation; transmembrane receptor protein tyrosine kinase activation (dimerization)