Western Blot (WB)
(Anti-ESRP2 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:78 kDa)
Rabbit anti-Human ESRP2 Polyclonal Antibody | anti-ESRP2 antibody
Anti-ESRP2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ESRP2; Polyclonal Antibody; Anti-ESRP2 Antibody; Rabbit Polyclonal; ESRP2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-PP7059 Antibody; anti-ESRP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ESRP2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ESRP2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human ESRP2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ESRP2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ESRP2 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:78 kDa)
Western Blot (WB)
(Anti-ESRP2 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:78 kDa)
Related Product Information for anti-ESRP2 antibody
Epithelial splicing regulatory protein 2 (ESRP2) is a conserved regulatory factor that controls the neonatal-to-adult switch of ~20% of splice isoforms in mouse and human hepatocytes. The normal shift in splicing coincides tightly with dramatic postnatal induction of ESRP2 in hepatocytes. Forced expression of ESRP2 in immature mouse and human hepatocytes is sufficient to drive a reciprocal shift in splicing and causes various physiological abnormalities. ESRP2 deletion in mice causes excessive hepatocyte proliferation upon injury, whereas forced expression of ESRP2 inhibits proliferation by suppressing the expression of neonatal Hippo pathway isoforms. ESRP2 and its closely related isoform ESRP1 are crucial to maintain an epithelial-specific RNA splicing program. Loss of these splicing factors leads to epithelial-to-mesenchymal transition (EMT) which is an important process involved in development, tumor progression, malignant transformation, and metastasis formation. In carcinogenesis, ESRPs appear to have a context-dependent dual function. Both isoforms have been found up-or downregulated in human cancers, and both high and low expression levels were associated with poor prognosis.
References
Munkley J, et al. (2019) Androgen-regulated transcription of esrp2 drives alternative splicing patterns in prostate cancer. Elife 8: e47678.Heilmann K, et al. (2017) Genome-wide screen for differentially methylated long noncoding rnas identifies esrp2 and lncrna esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer. Oncogene 36 (46): 6446-6461.Bhate A, et al. (2015) Esrp2 controls an adult splicing programme in hepatocytes to support postnatal liver maturation. Nat Commun 6 8768.
Similar Products
Product Notes
The ESRP2 (Catalog #AAA8109748) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ESRP2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ESRP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ESRP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESRP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
