Western Blot (WB)
(Anti-TMEM199 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lane C: U 251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)
Rabbit anti-Human TMEM199 Polyclonal Antibody | anti-TMEM199 antibody
Anti-TMEM199 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
TMEM199; Polyclonal Antibody; Anti-TMEM199 Antibody; Rabbit Polyclonal; TMEM199 Antibody; Rabbit PAb; Antigen Affinity Purified; transmembrane protein 199; Anti-C17orf32 Antibody; anti-TMEM199 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TMEM199
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TMEM199 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human TMEM199 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TMEM199 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-TMEM199 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lane C: U 251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)
Western Blot (WB)
(Anti-TMEM199 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lane C: U 251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)
Immunoprecipitation (IP)
(TMEM199 was immunoprecipitated using: Lane A:0.5 mg U 251 MG Whole Cell Lysate 4 uL anti-TMEM199 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TMEM199 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa Observed band size :23 kDa)
Immunoprecipitation (IP)
(TMEM199 was immunoprecipitated using: Lane A:0.5 mg U 251 MG Whole Cell Lysate 4 uL anti-TMEM199 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TMEM199 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa Observed band size :23 kDa)
Related Product Information for anti-TMEM199 antibody
TMEM199 (Transmembrane Protein 199) is a Protein Coding gene. The TMEM199 gene, located on 17q11.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. TMEM199 is a protein involved in Golgi homeostasis. The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. The encoded protein shares some homology with the yeast protein Vma12. TMEM199 is widely expressed in the kidney, thyroid, and other tissues. Diseases associated with TMEM199 include Congenital Disorder Of Glycosylation, Type lip, and Immunodeficiency 47.
References
Swayampakula M, et al. (2017) The interactome of metabolic enzyme carbonic anhydrase ix reveals novel roles in tumor cell migration and invadopodia/mmp14-mediated invasion. Oncogene 36 (45): 6244-6261.Kwan JS, et al. (2014) Meta-analysis of genome-wide association studies identifies two loci associated with circulating osteoprotegerin levels. Hum Mol Genet 23 (24): 6684-6693.Jansen JC, et al. (2016) Tmem199 deficiency is a disorder of golgi homeostasis characterized by elevated aminotransferases, alkaline phosphatase, and cholesterol and abnormal glycosylation. Am J Hum Genet 98 (2): 322-330.
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Product Notes
The TMEM199 (Catalog #AAA8108212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMEM199 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM199 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the TMEM199 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM199, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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