Western Blot (WB)
(Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Arp3/ACTR3 Polyclonal Antibody | anti-ACTR3 antibody
Anti-Arp3/ACTR3 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
ICC/IF: 1:300-1:10,000
IP: 1-4uL/mg of lysate
Western Blot (WB)
(Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ACTR3 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of ACTR3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ACTR3 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of ACTR3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ACTR3 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunoprecipitation (IP)
(ACTR3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-ACTR3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ACTR3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 47 kDaObserved band size: 47 kDa)
Immunoprecipitation (IP)
(ACTR3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-ACTR3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ACTR3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 47 kDaObserved band size: 47 kDa)
NCBI and Uniprot Product Information
NCBI Description
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
Uniprot Description
Arp3: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Belongs to the actin family. ARP3 subfamily.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2q14.1
Cellular Component: Golgi membrane; Arp2/3 protein complex; focal adhesion; hemidesmosome; membrane; lamellipodium; excitatory synapse; actin filament; podosome; intercellular junction; cytosol; actin cytoskeleton
Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; ATP binding
Biological Process: positive regulation of filopodium formation; meiotic chromosome movement towards spindle pole; axon guidance; regulation of myosin II filament assembly or disassembly; establishment and/or maintenance of cell polarity; positive regulation of dendrite morphogenesis; cytokinesis after meiosis; response to carbohydrate stimulus; spindle localization; response to antibiotic; asymmetric cell division; ephrin receptor signaling pathway; innate immune response; cell motility
Research Articles on ACTR3
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Product Notes
The ACTR3 actr3 (Catalog #AAA8107529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Arp3/ACTR3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Arp3/ACTR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 ICC/IF: 1:300-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the ACTR3 actr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arp3/ACTR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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