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Western Blot (WB) (Western blot analysis using LCN1 mouse mAb against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate (2).)

Mouse anti-Human LCN1 Monoclonal Antibody | anti-LCN1 antibody

LCN1 Antibody

Gene Names
LCN1; TP; TLC; PMFA; VEGP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
LCN1; Monoclonal Antibody; LCN1 Antibody; LCN1_HUMAN; Lipocalin 1 (protein migrating faster than albumin; tear prealbumin); Lipocalin 1 like 2; Lipocalin-1; PMFA; Tear lipocalin; Tear prealbumin; Tlc; TP; VEG protein; VEGP; Von Ebner gland protein; anti-LCN1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
LCN1 antibody detects endogenous levels of total LCN1
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
176
Applicable Applications for anti-LCN1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000

Optimal dilutions/concentrations should be determined by the end user.
Immunogen
Purified recombinant fragment of human LCN1 expressed in E. Coli
Subcellular Location
Secreted.
Tissue Specificity
Mainly expressed in lachrymal and salivary glands. Also expressed in the prostate.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis using LCN1 mouse mAb against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate (2).)

Western Blot (WB) (Western blot analysis using LCN1 mouse mAb against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate (2).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human prostate tissues using LCN1 mouse mab.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human prostate tissues using LCN1 mouse mab.)

Immunofluoresence/Immunocytochemistry (IF/ICC)

(Confocal immunofluorescence analysis of methanol-fixed HEK293 cells transfected with LCN1-hlgGFc using LCN1 mouse mAb(greeen), showing membrance localization. Blue: DRAQ5 fluorescent DNA dye.)

Immunofluoresence/Immunocytochemistry (IF/ICC) (Confocal immunofluorescence analysis of methanol-fixed HEK293 cells transfected with LCN1-hlgGFc using LCN1 mouse mAb(greeen), showing membrance localization. Blue: DRAQ5 fluorescent DNA dye.)
Related Product Information for anti-LCN1 antibody
LCN1: lipocalin 1 (tear prealbumin), also known as TP, PMFA, VEGP. Entrez Protein: NP_002288. The protein belongs to the lipocalin family. Lipocalins are a group of extracellular proteins that are able to bind lipophiles by enclosure within their structures to minimize solvent contact. This protein may bind hydrophobic ligands and inhibit cysteine proteinases. It may also play a role in taste reception and could be necessary for the concentration and delivery of sapid molecules in the gustatory system.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19.2 kDa
NCBI Official Full Name
lipocalin-1 isoform 1
NCBI Official Synonym Full Names
lipocalin 1
NCBI Official Symbol
LCN1
NCBI Official Synonym Symbols
TP; TLC; PMFA; VEGP
NCBI Protein Information
lipocalin-1
UniProt Protein Name
Lipocalin-1
Protein Family
UniProt Gene Name
LCN1
UniProt Synonym Gene Names
VEGP; Tlc; TP; VEG protein

NCBI Description

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.

Research Articles on LCN1

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Product Notes

The LCN1 lcn1 (Catalog #AAA9602712) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LCN1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LCN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the LCN1 lcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LCN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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