Arp3/ACTR3 Recombinant Protein | ACTR3 recombinant protein

Arp3/ACTR3 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121823

• Gene Names: ACTR3; ARP3
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Arp3/ACTR3; Arp3/ACTR3 Protein; Human; Recombinant (His & GST Tag); Human ACTR3 Protein (His & GST Tag); ARP3 Protein; ARP3 actin-related protein 3 homolog (yeast); ACTR3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol, 3mM DTT. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Ser418

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human ACTR3 (NP_005712.1)(Ala2- Ser418) was expressed with a N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5031573
• NCBI GeneID: 10096
• NCBI Accession #: NP_005712.1
• NCBI GenBank Nucleotide #: NM_005721.4
• UniProt Accession #: P61158; P32391; Q53QM2
• Molecular Weight: 47,371 Da
• NCBI Official Full Name: actin-related protein 3 isoform 1
• NCBI Official Synonym Full Names: ARP3 actin-related protein 3 homolog (yeast)
• NCBI Official Symbol: ACTR3
• NCBI Official Synonym Symbols: ARP3
• NCBI Protein Information: actin-related protein 3; actin-like protein 3
• UniProt Protein Name: Actin-related protein 3
• UniProt Gene Name: ACTR3
• UniProt Synonym Gene Names: ARP3
• UniProt Entry Name: ARP3_HUMAN

NCBI Description

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]

Uniprot Description

Arp3: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Belongs to the actin family. ARP3 subfamily.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: Golgi membrane; Arp2/3 protein complex; focal adhesion; hemidesmosome; membrane; lamellipodium; excitatory synapse; actin filament; podosome; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; ATP binding

Biological Process: positive regulation of filopodium formation; meiotic chromosome movement towards spindle pole; axon guidance; regulation of myosin II filament assembly or disassembly; establishment and/or maintenance of cell polarity; positive regulation of dendrite morphogenesis; cytokinesis after meiosis; response to carbohydrate stimulus; spindle localization; response to antibiotic; asymmetric cell division; ephrin receptor signaling pathway; innate immune response; cell motility