Mouse anti-Human UBE2W Monoclonal Antibody | anti-UBE2W antibody

Anti-UBE2W Antibody, Mouse Monoclonal

Cat. Num. AAA8106588

• Gene Names: UBE2W; UBC16; UBC-16
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A
• Synonyms: UBE2W; Monoclonal Antibody; Anti-UBE2W Antibody; Mouse Monoclonal; UBE2W Antibody; Mouse MAb; ubiquitin-conjugating enzyme E2W (putative); Anti-UBC-16 Antibody; Anti-UBC16 Antibody; anti-UBE2W antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 04
• Specificity: Human UBE2W
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UBE2W antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Recombinant Human UBE2W protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human UBE2W (rh UBE2W). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-UBE2W mouse monoclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)

Related Product Information for anti-UBE2W antibody

Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.

References

Zhang Y, et al. (2008) Generation of mouse UBE2W antibody and analysis of UBE2W expression in mouse tissues. Sheng Wu Gong Cheng Xue Bao. 24(4): 547-52.Scaglione KM, et al. (2011) Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell. 43(4): 599-612.Zhang Y, et al. (2011) UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2. Mol Cells. 31(2): 113-22.

NCBI and Uniprot Product Information

• NCBI GI #: 402169567
• NCBI GeneID: 55284
• NCBI Accession #: NP_001001481.2
• NCBI GenBank Nucleotide #: NM_001001481.2
• UniProt Accession #: Q96B02; Q1XBE0; Q9H823; Q9HAG6; Q9NV07; B4DIV1
• Molecular Weight: 20,689 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 W isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2W (putative)
• NCBI Official Symbol: UBE2W
• NCBI Official Synonym Symbols: UBC16; UBC-16
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 W; N-terminus-conjugating E2; ubiquitin carrier protein W; ubiquitin-conjugating enzyme 16; ubiquitin-conjugating enzyme 2W; ubiquitin-protein ligase W
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 W
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2W
• UniProt Synonym Gene Names: UBC16; UBC-16
• UniProt Entry Name: UBE2W_HUMAN

NCBI Description

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; misfolded or incompletely synthesized protein catabolic process; DNA repair