Rabbit anti-Mouse ErbB4 Polyclonal Antibody | anti-ERBB4 antibody

Anti-ErbB4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103470

• Gene Names: Erbb4; Her4; c-erbB-4
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: ErbB4; Polyclonal Antibody; Anti-ErbB4 Antibody; Rabbit Polyclonal; ErbB4 Antibody; Rabbit PAb; Antigen Affinity Purified; erb-b2 receptor tyrosine kinase 4; Anti-c-erbB-4 Antibody; Anti-Her4 Antibody; anti-ERBB4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse ErbB4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ERBB4 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Mouse ErbB4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse ErbB4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse ERBB4 in mouse stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse ERBB4 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-ERBB4 antibody

ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

Product Categories/Family for anti-ERBB4 antibody

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Huang, Y Z, et al. (2000) Regulation of neuregulin signaling by PSD-95 interacting with ErbB4 at CNS synapses. Neuron. 26(2):443-55.
Garcia, R A, et al. (2000) The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses. Proc Natl Acad Sci. 97(7):3596-601.
Silberberg G, et al. (2006) The involvement of ErbB4 with schizophrenia: association and expression studies. Am J Med Genet. 141(B2):142-8.
Sardi SP, et al. (2006) Presenilin-dependent ErbB4 nuclear signaling regulates the timing of astrogenesis in the developing brain. Cell. 127(1):185-97.

NCBI and Uniprot Product Information

• NCBI GI #: 146134398
• NCBI GeneID: 13869
• NCBI Accession #: NP_034284.1
• NCBI GenBank Nucleotide #: NM_010154.1
• UniProt Accession #: Q61527; O88460; Q3UNS6; B2KGF5; B2KGF6
• Molecular Weight: 146,855 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-4
• NCBI Official Synonym Full Names: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• NCBI Official Symbol: Erbb4
• NCBI Official Synonym Symbols: Her4; c-erbB-4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; avian erythroblastosis oncogene B 4; proto-oncogene-like protein c-ErbB-4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb4
• UniProt Synonym Gene Names: Mer4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_MOUSE

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; EGFR family

Cellular Component: mitochondrion; membrane; basolateral plasma membrane; postsynaptic density; cytoplasm; plasma membrane; integral to membrane; caveola; nucleus; receptor complex; lipid raft

Molecular Function: transferase activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; receptor signaling protein tyrosine kinase activity; ATP binding; protein kinase activity; epidermal growth factor receptor binding

Biological Process: lactation; synapse maturation; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid autophosphorylation; heart development; positive regulation of transcription, DNA-dependent; multicellular organismal development; signal transduction; embryonic pattern specification; regulation of cell migration; olfactory bulb interneuron differentiation; protein amino acid phosphorylation; positive regulation of glucose import; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; nervous system development; cell migration; cell fate commitment; transcription, DNA-dependent; positive regulation of synaptic transmission, GABAergic; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; mitochondrial fragmentation during apoptosis; surfactant homeostasis; phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; negative regulation of apoptosis