Mouse anti-Human TAF6 Monoclonal Antibody | anti-TAF6 antibody

Mouse monoclonal antibody Anti-Human TAF6

Cat. Num. AAA120136

• Gene Names: TAF6; TAF2E; TAFII70; TAFII80; TAFII85; MGC:8964; TAFII-70; TAFII-80; TAF(II)70; TAF(II)80
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot
• Synonyms: TAF6; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human TAF6; Homo sapiens TAF6 RNA polymerase II; TATA box binding protein (TBP)-associated factor; 80kDa (TAF6); transcript variant 1; TAF2E; TAFII70;TAFII80; TAFII85; MGC:8964; DKFZp781E21155; anti-TAF6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 585D4a
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 2787

• Applicable Applications for anti-TAF6 antibody: Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-TAF6 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of TAF6 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 585D4a-1]Predicted molecular weight: 72 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-TAF6 mAb. [Lot No. 585D4a-1]Predicted molecular weight: 72 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TAF6 mAb. [Lot No. 585D4a-1])

Related Product Information for anti-TAF6 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 298676521
• NCBI GeneID: 6878
• NCBI Accession #: NP_005632.1
• NCBI GenBank Nucleotide #: NM_005641
• Molecular Weight: 76,995 Da
• NCBI Official Full Name: Homo sapiens TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
• NCBI Official Symbol: TAF6
• NCBI Official Synonym Symbols: TAF2E; TAFII70; TAFII80; TAFII85; MGC:8964; TAFII-70; TAFII-80; TAF(II)70; TAF(II)80
• NCBI Protein Information: transcription initiation factor TFIID subunit 6; RNA polymerase II TBP-associated factor subunit E; TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD; transcription initiation factor TFIID 70 kDa subunit
• UniProt Protein Name: Transcription initiation factor TFIID subunit 6
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF6
• UniProt Entry Name: TAF6_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

TAF6: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Belongs to the TAF6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of cell proliferation; viral reproduction; regulation of transcription factor activity; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression; negative regulation of cell cycle