Rabbit anti-Human NOV/CCN3 Polyclonal Antibody | anti-NOV antibody

Anti-NOV/CCN3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102028

• Gene Names: NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: NOV/CCN3; Polyclonal Antibody; Anti-NOV/CCN3 Antibody; Rabbit Polyclonal; CCN3/NOV/IGFBP9 Antibody; Rabbit PAb; Antigen Affinity Purified; nephroblastoma overexpressed; Anti-CCN3 Antibody; Anti-IBP-9 Antibody; Anti-IGFBP-9 Antibody; Anti-IGFBP9 Antibody; Anti-NOVh Antibody; anti-NOV antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CCN3/NOV/IGFBP9
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NOV antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:1000-1:5000
• Immunogen: Recombinant Human CCN3/NOV/IGFBP9 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CCN3/NOV/IGFBP9 (rh CCN3/NOV/IGFBP9). CCN3/NOV/IGFBP9 specific IgG was purified by Human CCN3/NOV/IGFBP9 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human NOV in human brain with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC)

(Immunochemical staining of human NOV in human brain (from 2 donors) with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections.)

Western Blot (WB)

(Anti-NOV rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDaObserved band size:37 kDa)

Related Product Information for anti-NOV antibody

Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment.

References

Manara MC, et al. (2002) The expression of ccn3(nov) gene in musculoskeletal tumors. Am J Pathol. 160(3): 849-59.Lin CG, et al. (2003) CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family. J Biol Chem. 278(26): 24200-8.Vallacchi V, et al. (2009) CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. Cancer Res. 68(3): 715-23.Sin WC, et al. (2009) Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization. J Biol Chem. 284(43): 29935-44.McCallum L, et al. (2009) CCN3--a key regulator of the hematopoietic compartment. Blood Rev. 23(2): 79-85.

NCBI and Uniprot Product Information

• NCBI GI #: 4505423
• NCBI GeneID: 4856
• NCBI Accession #: NP_002505.1
• NCBI GenBank Nucleotide #: NM_002514.3
• UniProt Accession #: P48745; Q6I9S3; Q96BY5; Q9UDE4; B2R5X7
• Molecular Weight: 39,162 Da
• NCBI Official Full Name: protein NOV homolog
• NCBI Official Synonym Full Names: nephroblastoma overexpressed
• NCBI Official Symbol: NOV
• NCBI Official Synonym Symbols: CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• NCBI Protein Information: protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
• UniProt Protein Name: Protein NOV homolog
• Protein Family: Protein
• UniProt Gene Name: NOV
• UniProt Synonym Gene Names: CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
• UniProt Entry Name: NOV_HUMAN

NCBI Description

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]

Uniprot Description

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: proteinaceous extracellular matrix; intracellular membrane-bound organelle; cell soma; axon; dendrite

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction