Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) antibody)
Rabbit HER2 Polyclonal Antibody | anti-HER2 antibody
HER2 antibody
Gene Names
ERBB2; HER2; erb2
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
HER2 antibody was purified by immunoaffinity chromatography.
Synonyms
HER2; Polyclonal Antibody; HER2 antibody; Polyclonal HER2; Anti-HER2; herstatin; Phospho Tyr1248; tyrosine kinase r; tkr1; p185erbb2; Her-2 Neu; oncogene ngl; Her2/Neu; Her2 Neu; Her2; neu oncogene; HER-2; HER 2; her-2/neu; anti-HER2 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
HER2 antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
304
Applicable Applications for anti-HER2 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Biological Significance
HER2 is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340 (cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
HER2 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y(p)-L-G) derived from Human HER2 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1248) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1248) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-HER2 antibody
Rabbit polyclonal HER2 antibody
Product Categories/Family for anti-HER2 antibody
NCBI and Uniprot Product Information
Research Articles on HER2
Similar Products
Product Notes
The HER2 (Catalog #AAA835203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HER2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HER2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HER2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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