Rabbit anti-Human PAX5 Polyclonal Antibody | anti-PAX5 antibody

Anti-PAX5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100505

• Gene Names: PAX5; BSAP
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PAX5; Polyclonal Antibody; Anti-PAX5 Antibody; Rabbit Polyclonal; PAX5 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-BSAP Antibody; anti-PAX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PAX5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PAX5 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 4-6uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human PAX5
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PAX5, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PAX5 rabbit polyclonal antibody at 1:100 dilution Lane A: Daudi Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa (We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(PAX5 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL anti-PAX5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-PAX5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)

Related Product Information for anti-PAX5 antibody

Somatic alterations of the lymphoid transcription factor gene PAX5 (also known as BSAP) are a hallmark of B cell precursor acute lymphoblastic leukemia (B-ALL). PAX5 is a well-known haploinsufficient tumor suppressor gene in human B-cell precursor acute lymphoblastic leukemia (B-ALL) and is involved in various chromosomal translocations that fuse a part of PAX5 with other partners. PAX5, one of nine members of the mammalian paired box (PAX) family of transcription factors, plays an important role in B cell development. Approximately one-third of individuals with pre-B acute lymphoblastic leukemia (ALL) acquire heterozygous inactivating mutations of PAX5 in malignant cells, and heterozygous germline loss-of-function PAX5 mutations cause an autosomal dominant predisposition to ALL. PAX5 is expressed in the immune-privileged brain, B-lymphocytes, lymph nodes, and spleen.

References

Maurya SK, et al. (2018) Co-localization and interaction of pax5 with iba1 in brain of mice. Cell Mol Neurobiol 38 (4): 919-927.Jamrog L, et al. (2018) Pax5-eln oncoprotein promotes multistep b-cell acute lymphoblastic leukemia in mice. Proc Natl Acad Sci U S A 115 (41): 10357-10362.Hart MR, et al. (2018) Activating pax gene family paralogs to complement pax5 leukemia driver mutations. PLoS Genet 14 (9): e1007642.

NCBI and Uniprot Product Information

• NCBI GI #: 9951920
• NCBI GeneID: 5079
• NCBI Accession #: NP_057953.1
• NCBI GenBank Nucleotide #: NM_016734.1
• UniProt Accession #: Q02548; O75933; Q5SFM2; Q6S728; Q6S729; Q6S730; Q6S731; Q6S732; A3QVP6; A3QVP7; A3QVP8
• Molecular Weight: 42,149 Da
• NCBI Official Full Name: paired box protein Pax-5
• NCBI Official Synonym Full Names: paired box 5
• NCBI Official Symbol: PAX5
• NCBI Official Synonym Symbols: BSAP
• NCBI Protein Information: paired box protein Pax-5; paired box homeotic gene 5; transcription factor PAX 5; B cell specific activator protein; B-cell lineage specific activator; B-cell-specific transcription factor
• UniProt Protein Name: Paired box protein Pax-5
• Protein Family: Paired box protein
• UniProt Gene Name: PAX5
• UniProt Synonym Gene Names: BSAP
• UniProt Entry Name: PAX5_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PAX5: May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. Interacts with DAXX. Binds DNA as a monomer. Binds TLE4.

Protein type: Oncoprotein

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of histone H3-K9 methylation; nervous system development; organ morphogenesis; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; humoral immune response

Disease: Leukemia, Acute Lymphoblastic, Susceptibility To, 3