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Western Blot (WB) (Western blot analysis of RAB13 antibody (Center) in mouse bladder tissue lysates (35ug/lane). RAB13 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse RAB13 Polyclonal Antibody | anti-RAB13 antibody

RAB13, ID (Ras-related Protein Rab-13, Cell Growth-inhibiting Gene 4 Protein, GIG4)

Gene Names
RAB13; GIG4
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Purified
Purified by ammonium sulfate precipitation.
Synonyms
RAB13; Polyclonal Antibody; ID (Ras-related Protein Rab-13; Cell Growth-inhibiting Gene 4 Protein; GIG4); Anti -RAB13; anti-RAB13 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RAB13. Species Crossreactivity: mouse.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-RAB13 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunogen
Synthetic peptide selected from the Center region of human RAB13 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RAB13 antibody (Center) in mouse bladder tissue lysates (35ug/lane). RAB13 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RAB13 antibody (Center) in mouse bladder tissue lysates (35ug/lane). RAB13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB13 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB13 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using RAB13 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of 293 cells using RAB13 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RAB13 antibody
RAB13 can participate in polarized transport, in the assembly and/or the activity of tight junctions.
Product Categories/Family for anti-RAB13 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
22,774 Da
NCBI Official Full Name
RAB13, member RAS oncogene family
NCBI Official Synonym Full Names
RAB13, member RAS oncogene family
NCBI Official Symbol
RAB13
NCBI Official Synonym Symbols
GIG4
NCBI Protein Information
ras-related protein Rab-13; RAS-associated protein RAB13; growth-inhibiting gene 4 protein; cell growth-inhibiting gene 4 protein
UniProt Protein Name
Ras-related protein Rab-13
Protein Family
UniProt Gene Name
RAB13
UniProt Entry Name
RAB13_HUMAN

NCBI Description

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013]

Uniprot Description

RAB13: Could participate in polarized transport, in the assembly and/or the activity of tight junctions. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: Golgi apparatus; neuron projection; cytoplasmic vesicle membrane; tight junction; recycling endosome membrane; trans-Golgi network; recycling endosome; lamellipodium; endocytic vesicle; cytoplasm; plasma membrane; cytoplasmic vesicle; lateral plasma membrane

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; cellular response to insulin stimulus; metabolic process; cortical actin cytoskeleton organization and biogenesis; endosome transport; Rab protein signal transduction; endocytic recycling; neurite development

Research Articles on RAB13

Similar Products

Product Notes

The RAB13 rab13 (Catalog #AAA626658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB13, ID (Ras-related Protein Rab-13, Cell Growth-inhibiting Gene 4 Protein, GIG4) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RAB13 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the RAB13 rab13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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