Rabbit IL-15 Polyclonal Antibody | anti-IL15 antibody

Anti-IL-15 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100250

• Gene Names: Il15; IL-15; AI503618
• Reactivity: Mouse; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: IL-15; Polyclonal Antibody; Anti-IL-15 Antibody; Rabbit Polyclonal; IL15 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 15; Anti-AI503618 Antibody; anti-IL15 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse IL-15
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL15 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the mouse mIL15
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the mouse mIL15, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IL15 rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-IL15 antibody

The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.

Product Categories/Family for anti-IL15 antibody

AKT Signaling Pathway

References

Mongini PKA, Gupta R, Boyle E, et al. TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic lymphocytic leukemia B cells. Journal of immunology (Baltimore, Md: 1950). 2015;195(3):901-923.

NCBI and Uniprot Product Information

• NCBI GI #: 363000984
• NCBI GeneID: 16168
• NCBI Accession #: NP_001241676.1
• NCBI GenBank Nucleotide #: NM_001254747.1
• UniProt Accession #: P48346
• Molecular Weight: 18,593 Da
• NCBI Official Full Name: interleukin-15
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: Il15
• NCBI Official Synonym Symbols: IL-15; AI503618
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• Protein Family: Interleukin
• UniProt Gene Name: Il15
• UniProt Synonym Gene Names: IL-15
• UniProt Entry Name: IL15_MOUSE

Uniprot Description

IL15: Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell cycle regulation; Secreted; Secreted, signal peptide

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of immune response; NK T cell proliferation; negative regulation of smooth muscle cell proliferation; cell maturation; signal transduction; lymph node development; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; extrathymic T cell selection; natural killer cell differentiation; positive regulation of cell proliferation; immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; regulation of defense response to virus by host; hyaluronan metabolic process