Rabbit KRT10 Polyclonal Antibody | anti-KRT10 antibody

Anti-KRT10 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101839

• Gene Names: KRT10; BIE; EHK; K10; KPP; BCIE; CK10
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: KRT10; Polyclonal Antibody; Anti-KRT10 Antibody; Rabbit Polyclonal; KRT10 Antibody; Rabbit PAb; Antigen Affinity Purified; keratin 10; Anti-BCIE Antibody; Anti-BIE Antibody; Anti-CK10 Antibody; Anti-EHK Antibody; Anti-K10 Antibody; Anti-KPP Antibody; anti-KRT10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human KRT10
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-KRT10 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human KRT10
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human KRT10, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining KRT10 in skin with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining KRT10 in human breast carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining KRT10 in human lung cancer with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining KRT10 in mouse skin with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining KRT10 in rat skin with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-KRT10 antibody

KRT10 was identified as a differently expressed protein in cisplatin-treated C13K/PTEN cells. Further study confirmed that cisplatin could induce upregulation of KRT10 mRNA and protein in C13K/PTEN cells and there was a direct interaction between KRT10 and PTEN. KRT10 is a downstream molecule of PTEN which improves cisplatin-resistance of ovarian cancer and forced KRT10 overexpression may also act as a therapeutic method for overcoming MDR in ovarian cancer. The substitution of arginine (R) to histidine (H) at amino acid residue 156 (R156H) of the coiled 1A region is one of the most frequent mutations of KRT10. Ichthyosis with confetti (IWC) is a genodermatosis caused by dominant-negative mutations in the gene encoding keratin 10 (KRT10). Recombinant adenovirus-mediated overexpression of KRT10 and PTEN may improve the cisplatin resistance of ovarian cancer in vitro and in vivo.

References

Wu H, et al. (2015) Recombinant adenovirus-mediated overexpression of pten and krt10 improves cisplatin resistance of ovarian cancer in vitro and in vivo. Genet Mol Res 14 (2): 6591-6597.Wu H, et al. (2014) Pten overexpression improves cisplatin-resistance of human ovarian cancer cells through upregulating krt10 expression. Biochem Biophys Res Commun 444 (2): 141-146.Spoerri I, et al. (2015) The phenotypic and genotypic spectra of ichthyosis with confetti plus novel genetic variation in the 3' end of krt10: From disease to a syndrome. JAMA Dermatol 151 (1): 64-69.Mayuzumi N, et al. (2000) Recurrent r156h mutation of krt10 in a japanese family with bullous congenital ichthyosiform erythroderma. J Eur Acad Dermatol Venereol 14 (4): 304-306.

NCBI and Uniprot Product Information

• NCBI GI #: 195972866
• NCBI GeneID: 3858
• NCBI Accession #: NP_000412.3
• NCBI GenBank Nucleotide #: NM_000421.3
• UniProt Accession #: P13645; Q14664; Q8N175
• Molecular Weight: 58,827 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 10
• NCBI Official Synonym Full Names: keratin 10
• NCBI Official Symbol: KRT10
• NCBI Official Synonym Symbols: BIE; EHK; K10; KPP; BCIE; CK10
• NCBI Protein Information: keratin, type I cytoskeletal 10; CK-10; keratin-10; cytokeratin 10
• UniProt Protein Name: Keratin, type I cytoskeletal 10
• Protein Family: Keratin
• UniProt Gene Name: KRT10
• UniProt Synonym Gene Names: KPP; CK-10; K10
• UniProt Entry Name: K1C10_HUMAN

NCBI Description

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; membrane; keratin filament; cytoplasm; intermediate filament; nucleus

Molecular Function: structural constituent of epidermis

Biological Process: keratinocyte differentiation

Disease: Erythroderma, Ichthyosiform, Congenital Reticular; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis