Rabbit anti-Human Ki67/MKI67 Polyclonal Antibody | anti-MKI67 antibody

Anti-Ki67/MKI67 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100132

• Gene Names: MKI67; KIA
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Ki67/MKI67; Polyclonal Antibody; Anti-Ki67/MKI67 Antibody; Rabbit Polyclonal; Ki-67 Antibody; Rabbit PAb; Antigen Affinity Purified; marker of proliferation Ki-67; Anti-KIA Antibody; Anti-MIB-Antibody; Anti-MIB-1 Antibody; Anti-PPP1R105 Antibody; anti-MKI67 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MKI67
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MKI67 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the sequence of the human Ki-67
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the sequence of the human Ki-67., and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MKI67 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: SKBR3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:359 kDaObserved band size:359 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MKI67 antibody

MKI67 contains 1 FHA domain and plays a key role in cell proliferation. During interphase, the MKI67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. MKI67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells. MKI67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of MKI67-positive tumor cells is often correlated with the clinical course of cancer. It is also associated with ribosomal RNA transcription. Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis. The MKI67 protein is a nuclear and nucleolar protein, which is tightly associated with somatic cell proliferation. Antibodies raised against the human MKI67 protein paved the way for the immunohistological assessment of cell proliferation, particularly useful in numerous studies on the prognostic value of cell growth in clinical samples of human neoplasms. MKI67 protein expression is an absolute requirement for progression through the cell-division cycle. Recently, MKI67 is proved to be an independent prognostic factor for disease-free survival (HR 1.5-1.72) in multivariate analyses studies using samples from randomized clinical trials with secondary central analysis of the biomarker. MKI67 was not found to be predictive for long-term follow-up after chemotherapy. Nevertheless, high KI-67 was found to be associated with immediate pathological complete response in the neoadjuvant setting, with an LOE of II-B. MKI67 could be considered as a prognostic biomarker for therapeutic decision.

References

Rahmanzadeh R, et al. (2007) Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif. 40(3):422-30. Bullwinkel J, et al. (2006) Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. J Cell Physiol. 206(3):624-35. Schonk DM, et al. (1989) Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum Genet. 83(3):297-9. Endl E, et al., 2000, Exp Cell Res. Jun 15;257(2): 231-7.Luporsi E, et al., 2012, Breast Cancer Res Treat. 132(3): 895-915.Scholzen T, et al., 2000, J Cell Physiol. 182(3): 311-22.

NCBI and Uniprot Product Information

• NCBI GI #: 225543215
• NCBI GeneID: 4288
• NCBI Accession #: NP_001139438.1
• NCBI GenBank Nucleotide #: NM_001145966.1
• UniProt Accession #: P46013; Q5VWH2
• Molecular Weight: 358,694 Da
• NCBI Official Full Name: antigen KI-67 isoform 2
• NCBI Official Synonym Full Names: antigen identified by monoclonal antibody Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA
• NCBI Protein Information: antigen KI-67; proliferation-related Ki-67 antigen
• UniProt Protein Name: Antigen KI-67
• Protein Family: Proliferation marker protein
• UniProt Gene Name: MKI67
• UniProt Entry Name: KI67_HUMAN

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: cell proliferation; meiosis