Mouse FKBP51 Monoclonal Antibody | anti-FKBP51 antibody

FKBP51 Antibody

Cat. Num. AAA8001890

• Gene Names: FKBP5; P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10
• Reactivity: Human, Mouse, Rat, Rabbit, Dog, Hamster
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: FKBP51; Monoclonal Antibody; FKBP51 Antibody; Mouse Anti-Human FKBP51 Monoclonal IgG1; Clone Hi51B; AIG6 Antibody; FK506 binding protein 5 Antibody; FKBP5 Antibody; FKBP54 Antibody; HSP90 binding immunophilin Antibody; p54 Antibody; Pplase Antibody; Ptg10 Antibody; Rotamase Antibody; T cekk FK506 binding protein Antibody; anti-FKBP51 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Rabbit, Dog, Hamster
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: Hi51B
• Specificity: Detects ~51kDa.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 457

• Applicable Applications for anti-FKBP51 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000; ICC/IF: 1:1000
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP51
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Nucleus
• Certificate of Analysis: A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 ug total protein using WB analysis.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B . Tissue: MK cells. Species: Mouse. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Cells stained red. Courtesy of: the Hospital Henri Mondor, France.)

Western Blot (WB)

(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of FKBP51 protein using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-FKBP51 antibody

HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506-and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis-to trans-proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Product Categories/Family for anti-FKBP51 antibody

Cancer; Heat Shock; Cell Signaling; Protein Trafficking; Nuclear Import/Export

References

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201. 5. Hubler T.R. et al. (2003) Endocrinology 144(6): 2380-2387.

NCBI and Uniprot Product Information

• NCBI GI #: 2851536
• NCBI GeneID: 2289
• UniProt Accession #: Q13451
• NCBI Official Full Name: Peptidyl-prolyl cis-trans isomerase FKBP5
• NCBI Official Synonym Full Names: FKBP prolyl isomerase 5
• NCBI Official Symbol: FKBP5
• NCBI Official Synonym Symbols: P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP5
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP5
• UniProt Gene Name: FKBP5
• UniProt Synonym Gene Names: AIG6; FKBP51; PPIase FKBP5; 51 kDa FKBP; FKBP-51; FKBP-5; p54
• UniProt Entry Name: FKBP5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

FKBP5: Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Part of a heteromultimeric cytoplasmic complex with HSP90, HSP70 and steroid receptors. Dissociates from the complex when NR3C1 binds glucocorticoid. By androgen. Widely expressed, enriched in testis compared to other tissues. Inhibited by FK506 but not cyclosporin.

Protein type: EC 5.2.1.8; Isomerase; Chaperone

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; heat shock protein binding

Biological Process: protein peptidyl-prolyl isomerization; protein folding

Disease: Major Depressive Disorder