SDS-Page
OSTF1 recombinant protein
OSTF1, 1-217aa, Human, His tag, E Coli
Gene Names
OSTF1; OSF; SH3P2; bA235O14.1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
OSTF1; 1-217aa; Human; His tag; E Coli; Osteoclast stimulating factor 1; SH3P2; OSF; OSTF1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.5mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MSKPPPKPVK PGEGGQVKVF RALYTFEPRT PDELYFEEGD IIYITDMSDT NWWKGTSKGR TGLIPSNYVA EQAESIDNPL HEAAKRGNLS WLRECLDNRV GVNGLDKAGS TALYWACHGG HKDIVEMLFT QPNIELNQQN KLGDTALHAA AWKGYADIVQ LFLAKGARTD LRNIEKKLAF DMATNAACAS LLKKKQGTDA VRTLSNAEDY LDDEDSDLEH HHHHH
Sequence Length
217
Applicable Applications for OSTF1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for OSTF1 recombinant protein
Osteoclast-stimulating factor-1 (OSTF1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast (OCL) formation and bone resorption. OSTF1 is highly expressed in OCL and contains structural domains such as SH3 and ankyrin repeats, which are potentially involved in protein-protein interactions. OSTF1 is ubiquitously expressed analogous to other regulators of OCL activity such as c-Src or transforming growth factor beta. Recombinant human OSTF1 protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for OSTF1 recombinant protein
References
Kurihara N., et al. (2001) J Bio Chem. 276 (44):41035-9; Reddy S., et al. (1998) Cell Physiol. 177 (4):636-45.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25.1 kDa (225 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
OSTF1 protein
NCBI Official Synonym Full Names
osteoclast stimulating factor 1
NCBI Official Symbol
OSTF1
NCBI Official Synonym Symbols
OSF; SH3P2; bA235O14.1
NCBI Protein Information
osteoclast-stimulating factor 1
UniProt Protein Name
Osteoclast-stimulating factor 1
Protein Family
UniProt Gene Name
OSTF1
NCBI Description
Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM, Mar 2008]
Uniprot Description
Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.
Research Articles on OSTF1
Similar Products
Product Notes
The OSTF1 ostf1 (Catalog #AAA203303) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's OSTF1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the OSTF1 ostf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSKPPPKPVK PGEGGQVKVF RALYTFEPRT PDELYFEEGD IIYITDMSDT NWWKGTSKGR TGLIPSNYVA EQAESIDNPL HEAAKRGNLS WLRECLDNRV GVNGLDKAGS TALYWACHGG HKDIVEMLFT QPNIELNQQN KLGDTALHAA AWKGYADIVQ LFLAKGARTD LRNIEKKLAF DMATNAACAS LLKKKQGTDA VRTLSNAEDY LDDEDSDLEH HHHHH. It is sometimes possible for the material contained within the vial of "OSTF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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